The methods and results of a gas-liquid chromatographic analysis of bile acids in serum are presented. The analysis of bile acids in serum involves enzymatic hydrolysis (cholylglycine hydrolase), preparation of propionated methyl ester derivatives of bile acid and gas chromatographic procedure with 2. 5 % OV-1. Adequate separation of the individual bile acids, (cholic, chenodeoxycholic and deox...

Lactobacillus strains are a major part of the probiotics, microflora of the intestine and of fermented dairy products, and are found in a variety of environments. The aim of this study was to find out the ability of bile and acid tolerance and antibacterial properties of the twenty eight isolates of three group lactobacilli namely Lactobacillus plantarum, Lactobacillus casei and Lactobacillus d...

This study was performed to enumerate the total viable cell count of probiotic in five brands (A to E) of commercially cultured milk drinks that are available in the Malaysian market as well as to test their tolerance to various pH and bile concentrations by simulating the human gastrointestinal pH and bile concentration. The acid tolerance test was studied under pH 1.5 and 3.0 with 7.2 as cont...

background: probiotics and especially lactobacilli are among the most important components widely used in food technology. isolation and characterization of indigenous probiotics should be performed in native populations. objectives: in order to isolate lactobacillus spp. from iranian traditional lighvan cheese to be used as starters, 15 cheese samples were randomly selected and tested for tech...

Background: Probiotics and especially Lactobacilli are among the most important components widely used in food technology. Isolation and characterization of indigenous probiotics should be performed in native populations. OBJECTIVES: In order to isolate lactobacillus spp. from Iranian traditional Lighvan cheese to be used as starters, 15 cheese samples were randomly selected and tested for tech...

The present investigation represents the effect of freeze drying on some properties as acid and bile tolerance of Streptococcus thermophilus MTCC 1938 culture isolated from dairy products. The cell paste obtained from milk based medium was freeze dried with a pressure of 50-100 mtorr for 24h at -40°C. Acid and bile tolerance test exhibited 3.8-4.9 and 3.2-3.8 log counts reduction after freeze d...

Nowadays, there is increasing interest in the production of functional foods, particularly probiotic foods. Lactic acid bacteria (LAB) particularly strains of Lactobacillus are important bacteria in food microbiology and human nutrition due to their ability to fermented food production and have received considerable attention as probiotics. The traditional fermented dairy foods as a rich source...

Nowadays, there is increasing interest in the production of functional foods, particularly probiotic foods. Lactic acid bacteria (LAB) particularly strains of Lactobacillus are important bacteria in food microbiology and human nutrition due to their ability to fermented food production and have received considerable attention as probiotics. The traditional fermented dairy foods as a rich source...

Resistance to bile acids is a necessary ability for bacteria that live in the gastrointestinal tract, such as Helicobacter pylori. The role of bacterial drug efflux system important acid tolerance. Using H. pylori JP26 strain and JP26AcrB deficient AcrB relationship between tolerance was investigated. Bacterial susceptibility measured by agar dilution method. To examine acids, intracellular acc...