Abstract A simple and sensitive method was proposed for choline, carnitine, acetylcarnitine (ACa) acetylcholine (ACh) separation in feed, blood urine of animals by using ion chromatography (IC) detection tandem mass spectrometry (MS/MS). Analytes were extracted a mixture acetonitrile water, purified C18 solid-phase extraction columns, separated via IC with an IonPac SCS-1 column detected MS/MS ...

Quercetin mainly exists in the skin of colored fruits and vegetables as one of flavonoids. Recent studies show that quercetin, like other flavonoids, has diverse pharmacological actions. However, relatively little is known about quercetin effects in the regulations of ligand-gated ion channels. In the previous reports, we have shown that quercetin regulates subsets of homomeric ligand-gated ion...

two cu(ii) complexes, [cu(l1)2](no3)2 (1) and [cu(l2)2] (no3)2(2), which l1 and l2 schiff base ligands are 2-(thiophene-2-yl) -1h-imidazo [4,5-f] [1,10] phenanthroline and 2-(furane-2-yl)-1h-imidazo[4,5-f] [1,10] phenenthroline, respectively, were synthesized and characterized by physico - chemical and spectroscopic methods. corresponded cuo nanoparticles were prepared by calcination of the obt...

Abstract Introduction Acetylcholine (ACh) spasm provocation testing together with the diagnostic criteria published by Coronary Vasomotor Disorders International Study Group (COVADIS) are considered gold standard for diagnosis of coronary vasospasm in contemporary clinical practice. In 20–29% patients undergoing an equivocal test result occurs when these only partially met and poses a dilemma. ...

Objective(s): Cholinergic neurons are important neurons in the Pedunculopontine tegmental nucleus (PPT). In this study, nicotinic receptor of the PPT in central cardiovascular regulation in the anesthetized rat was evaluated. Materials and Methods: Saline, acetylcholine (Ach; doses: 90 and 150 nmol), hexamethonium (Hexa; doses: 100 and 300 nmol) and higher doses of Hexa (300 nmol) + Ach (150 nm...

BACKGROUND/AIMS Oxidative stress induced intracellular Ca2+ overload plays an important role in the pathophysiology of several heart diseases. Acetylcholine (ACh) has been shown to suppress reactive oxygen species generation during oxidative stress. However, there is little information regarding the effects of ACh on the intracellular Ca2+ regulation in the presence of oxidative stress. Therefo...

Objective(s):To determine the effect of acetylcholine (ACh), pilocarpine, and atropine on pain evoked responses of pain excited neurons (PEN) and pain inhibited neurons (PIN) in hippocampal CA3 region of morphine addicted rats. Materials and Methods:Female Wistar rats, weighing between 230-260 g were used in this study. Morphine addicted rats were generated by subcutaneous injection of increasi...

objective(s):to determine the effect of acetylcholine (ach), pilocarpine, and atropine on pain evoked responses of pain excited neurons (pen) and pain inhibited neurons (pin) in hippocampal ca3 region of morphine addicted rats. materials and methods:female wistar rats, weighing between 230-260 g were used in this study. morphine addicted rats were generated by subcutaneous injection of increasi...

The tz31]~4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca 2+ channel. ¢z3/134-nAChR-expressing clones were identified using the fura-2 Ca 2÷ imaging technique, and were further characterised by single-cell and whole-cell patch-clamp studies. Acetylcholine (ACh) induced fast activat...