The inflorescences of ten species of the genus Jatropha were fixed in Cornoy’s fluid (6:3:1). Acetocarmine stain (2%) was used for staining the pollen mother cells. Seven species exhibited 11 bivalents and 2n =22 and x=11. But the two other species, J.villosa var. villosa and J.villosa var. ramnadensis showed only 10 bivalents and 2n number of 20 chromosomes and x=10. The study concluded the oc...

Embryogenic cultures of Araucaria angustifolia were established in a BM liquid medium supplemented with 2 μM 2,4-dichlorophenoxyacetic acid, 1 μM 6-benzylaminopurine and 1 μM kinetin (BM2) and in a BM medium free of growth regulators (BM0). During 42 days in culture, the cell growth pattern of both cultures was similar. The pH of the culture medium of both BM0 and BM2 underwent progressive redu...

Chromosomes were studied in somatic cells of 60 plants of blue grama, Bouteloua gracilis (Willd. ex Kunth) Lag ex Griffiths, collectedfrom a 7-hectarefield ofnative range at the Central Plains Experiment Range (CPER), Nunn, Colorado, to determine chromosome constitution in relation to the plant characteristics. Somatic chromosomes were studied in root tips collected from vigorously growingplant...

ABSTRACT Elaeis oleifera chromosomes are similar to those of E. guineensis, with close gene pools for the production interspecific O x G hybrids. The pollen viability and germination ‘Coarí’ guineensis ‘La Mé’ were compared their hybrid (‘Coarí’ Mé’). was determined by acetocarmine staining method (0.5 %) in vitro incubation on agar-sucrose medium (1.2-11.0 g 100 mL distilled water). significan...

Abstract To determine the optimal embryogenic capacity (somatic embryo production) of selected elite nematode-resistant genotypes Pinus thunbergii , variables such as tissue (ET) morphology, maternal genotype, proliferation rate and age were analyzed. ET morphology histological evaluation stage showed a decrease in filamentous clump protuberant surfaces decline acetocarmine-staining area, which...

Objective: To determine the stigma receptivity and pollen viability in order to make manual crosses obtain viable progeny castor bean (Ricinus communis L.).Design/Methodology/Approach: Pollen tests were made on breeding materials by staining with acetocarmine solution. In elite materials, of was evaluated hydrogen peroxide. From inflorescences, male ones removed female preserved, which covered ...

Pollen studies act as a prerequisite for choosing compatible pollen parent and help plant breeders to achieve successful crossing between different cultivars. An experiment was conducted during 2018–19 at Fruit Research Farm of Regional Horticulture Training Station Bajaura, Kullu, Himachal Pradesh evaluate 8 commercial genetically distant pomegranate (Punica granatum L.) cultivars (Chawla, Nab...

The objective of this study was to evaluate genetic stability by determining the meiotic index (MI) and pollen viability in F1RC2 segregating populations, obtained crosses between wheat commercial cultivars synthetic wheat, aimed at introgression/genetic resistance. For MI, three ears were collected per genotype, before anthesis, they fixed Carnoy’s solution. cytological slides prepared crushin...