BACKGROUND Making peptide pharmaceuticals involves challenging processes where many barriers, which include production and manufacture, need to be overcome. A non common but interesting research area is related to peptides with intracellular targets, which opens up new possibilities, allowing the modulation of processes occurring within the cell or interference with signaling pathways. However,...

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Label-free detection of the interaction between hexahistidine-tagged human κ-opioid receptor membrane protein and anti-His antibody is demonstrated in liquid by an optical microelectromechanical system utilizing electromagnetically actuated microresonators. Shift in resonance frequency due to accretion of mass on the sensitive surface of microresonators is monitored via an integrated optical re...

We evaluate the ability of hexahistidine (His6) tags on peptide and protein substrates to recruit deoxyribozymes for modifying those substrates. For two different deoxyribozymes, one that creates tyrosine-RNA nucleopeptides and another that phosphorylates tyrosine side chains, we find substantial improvements in yield, kobs, and Km for peptide substrates due to recruiting by His6/Cu(2+). Howeve...

Understanding multi-dimensional mechanism design. 1 The Model Let M = {1, . . . , m} be a finite set of agents. Every agent has private information, which can be multi-dimensional. This is called his type. The space from which an agent draws his type is called his type space. Let Ti denote the type space of agent i ∈ M . We assume Ti ⊆ R n for some integer n ≥ 1. Let T m = ×mi=1Ti. Also, denote...

We present a molecular theory to study the adsorption of different species within pH-sensitive hydrogel nanofilms. The theoretical framework allows for a molecular-level description of all the components of the system, and it explicitly accounts for the acid-base equilibrium. We concentrate on the adsorption of hexahistidine, one of the most widely used tags in bio-related systems, particularly...

In a group signature scheme, group members are able to sign messages on behalf of the group. Moreover, resulting signatures are anonymous and unlinkable for every verifier except for a given authority. In this paper, we mainly focus on one of the most secure and efficient group signature scheme, namely XSGS proposed by Delerablée and Pointcheval at Vietcrypt 2006. We show that it can efficientl...

The paper presents an off-line signature verification system using support vector machine technique. Global features are extracted from the signatures using radon transform. For each registered user in the system database a number of reference signatures are enrolled and aligned for statistics information extraction about his signature. Dynamic time warping algorithm is used to align two signat...

The polar flagellum of Vibrio alginolyticus is rotated by the sodium motor. The stator unit of the sodium motor consists of four different proteins: PomA, PomB, MotX and MotY. MotX and MotY, which are unique components of the sodium motor, form the T-ring structure attached to the LP ring in the periplasmic space. MotY has a putative peptidoglycan-binding motif in its C-terminal region and MotX...

The recombinant expression of soluble proteins in Escherichia coli continues to be a major bottleneck in structural genomics. The establishment of reliable protocols for the performance of small-scale expression and solubility testing is an essential component of structural genomic pipelines. The SSGCID Protein Production Group at the University of Washington (UW-PPG) has developed a high-throu...