introduction: acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. infection with acanthamoeba spp. poses threat to human health, such as, acanthamoeba keratitis (ak) that is a vision-threatening infection of the cornea. this study aimed to identify the species of acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain react...

In order to determine the feasibility of using radioactive precursors as markers for membrane phospholipids in Acanthamoeba palestinensis, the characteristics of phospholipids labeled with choline-14 C and glycerol-3H were examined . Choline-14 C was found to be a specific label for phosphatidyl choline . There was a turnover of the radioactive moiety of phosphatidyl choline at a rate that vari...

We investigated the value of mitochondrial small subunit rRNA gene (mt SSU rDNA) PCR-RFLP as a taxonomic tool for Acanthamoeba isolates with close inter-relationships. Twenty-five isolates representing 20 species were included in the analysis. As in nuclear 18S rDNA analysis, two type strains (A. astronyxis and A. tubiashi) of morphological group 1 diverged earliest from the other strains, but ...

The membranes of Acanthamoeba palestinensis were studied by examination in fixed cells, and then by following the movements of glycerol-(3)H-labeled phospholipids by cell fractionation. Two previously undescribed structures were observed: collapsed cytoplasmic vesicles of cup shape, and plaques in food vacuole and plasma membrane similar in size to the collapsed vesicles. It appeared that the p...

al. Burden of disease caused by Strepto-coccus pneumoniae in children younger than 5 years: global estimates. al. Dried blood spot real-time polymerase chain reaction assays to screen newborns for congenital cytomegalovirus infection.rial load in children and adolescents with pneumococcal pneumonia and empyema. cation of pneumococcal serotypes from culture-negative clinical specimens by novel r...

The survival of Salmonella enterica was recently shown to increase when the bacteria were sequestered in expelled food vacuoles (vesicles) of Tetrahymena. Because fresh produce is increasingly linked to outbreaks of enteric illness, the present investigation aimed to determine the prevalence of protozoa on spinach and lettuce and to examine their interactions with S. enterica, Escherichia coli ...

The free-living amoeba (FLA) group of protozoa that can be found in various types conditions nature, one which is the genus Acanthamoeba. Although, number infections caused by this protozoan low, diagnosis was still difficult to confirm and therefore there were a higher mortality, especially those associated with encephalitis. This review presents some information about biology, pathogenesis, c...