We have developed a method for measuring the electrophoretic mobility of submicrometer, fluorescently labeled particles and the electroosmotic mobility of a microchannel. We derive explicit expressions for the unknown electrophoretic and the electroosmotic mobilities as a function of particle displacements resulting from alternating current (AC) and direct current (DC) applied electric fields. ...

Nanopores have emerged as promising next-generation devices for DNA sequencing technology. The two major challenges in such devices are: (i) find an efficient way to raise the DNA capture rate prior to funnelling a nanopore, and (ii) reduce the translocation velocity inside it so that single base resolution can be attained efficiently. To achieve these, a novel soft nanopore comprising a solid-...

We analyze heat transfer characteristics of steady electroosmotic flows with an arbitrary pressure gradient in two-dimensional straight microchannels considering the effects of Joule heating in electroosmotic pumping. Both the temperature distribution and local Nusselt number are mathematically derived in this study. The thermal analysis takes into consideration of the interaction among advecti...

Tailor-designed alternating current electroosmotic (AC-EO) stagnation flows are used to convect bioparticles globally from a bulk solution to localized dielectrophoretic (DEP) traps that are aligned at the flow stagnation points. The multiscale trap, with a typical trapping time of seconds for a dilute 70 microL volume of 10(3) particles per cc sample, is several orders of magnitude faster than...

Dielectrophoretic assembly of nanoparticles has great utility in creating intricate designer nanoscale structures. Dielectrophoresis of gold nanoparticles has been used to create nanowires for electronics, high surface area electrodes for sensors, and nanoelectrode nanogaps for molecular electronics. Despite the utility of this technique, little is known about the mechanisms governing different...

Molecular dynamics simulation is applied to study the electroosmotic flow in rough nanochannels, with particular attention given to the fluid–solid interactions. In the simulation, the surface roughness is characterized by a fractal Cantor. The roles of roughness height and fractal dimension on nanoscale electroosmotic flow are examined and analyzed. The concentration distributions, zeta potent...

We present the design, test and theoretical analysis of a novel micropump. The purpose is to make a pump with large flow rate (approximately 10 microL min-1) and high pressure capacity (approximately 1 bar) powered by a low voltage DeltaV<30 V. The pump is operated in AC mode with an electroosmotic actuator in connection with a full wave rectifying valve system. Individual valves are based on a...

This paper reports on the fabrication and experimental characterisation of improved design AC eletroosmotic micropumps. Since the first study and analysis of AC electroosmosis by Ramos et al. [1], many devices have been fabricated and tested [2]. The basic planar electrode design was improved and increased pumping velocities were measured. Among the proposed improvements, 3D electrode design se...

This paper describes the estimation of binding constants (Kb) between carbonic anhydrase B (CAB, EC 4.2.1.1, from bovine erythrocytes) and charged benzenesulfonamides by affinity capillary electrophoresis (ACE) under conditions in which the migration time is affected by changes in electroosmotic flow and by nonspecific interactions accompanying changes in the concentration of ligand. Comparison...