The peroxidative activity of Cu,Zn-containing superoxide dismutase (Cu, Zn-SOD) was studied by using a chromogen, 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulfonate) (ABTS) which reacts with .OH radicals to form ABTS+, and the spin traps, N-tert-butyl-alpha-phenylnitrone (PBN) and 5,5-dimethyl-1-pyrroline N-oxide (DMPO). The formation of ABTS+. in this study required both active Cu,Zn-SOD and H2O...

Purpose: To improve the aqueous solubility of phloridzin by complexing it with hydroxypropyl-βcyclodextrin (HP-β-CD). Methods: The complex of phloridzin with HP-β-CD was prepared by freeze-drying method. The physicochemical properties of the complex were investigated by ultraviolet-visible spectrometry (UV), infrared spectrometry (IR), differential scanning calorimetry (DSC) and x-ray diffracto...

Laccase-mediator systems (LMS) have been widely studied for their capacity to oxidize the nonphenolic subunits of lignin (70-90% of the polymer). The phenolic subunits (10-30% of the polymer), which can also be oxidized without mediators, have received considerably less attention. Consequently, it remains unclear to what extent the presence of a mediator influences the reactions of the phenolic...

A sequential injection system based on the ABTS (2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic-acid) methodology was developed. The proposed method, incorporating a mixing chamber in the side port of the selection valve, was evaluated to measure the total antioxidant activity of several beverages and foods. The ABTS(+) is generated by oxidation of ABTS with potassium persulfate and is redu...

Radical scavenging and antioxidant activities of nine flavonoids previously isolated from the aerial parts of Chenopodium bonus-henricus L. were established using DPPH and ABTS free radicals as well as inhibition of lipid peroxidation in a linoleic acid system by the ferric thiocyanate method. Patuletin glycosides, compounds 1, 2 and 7 showed the highest DPPH (85.78%, 85.59%, 86.07%) and ABTS a...

Laccase-catalyzed oxygen delignification of kraft pulp offers some potential as a replacement for conventional chemical bleaching and has the advantage of requiring much lower pressure and temperature. However, chemical mediators are required for effective delignification by laccase, and their price is currently too high at the dosages required. To date, most studies have employed laccase from ...

Optimization of phenolic extraction from Orthosiphon stamineus was carried out in present study by investigating the effects of ethanol concentration (0-100%, v/v), extraction time (60-300 min) and extraction temperature (25-65oC) on the phenolic recovery using single factor experiment. Total phenolic content (TPC), total flavonoid content (TFC) and condensed tannins content (CTC) were used for...

Antioxidant activities and Anti-inflammatory activity of the extracts were evaluated by a 1, 1-diphenyl, 2-picrylhydrazyl free radical (DPPH), 2, 2’-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and antilipoxygenase assay for the water extraction of Thespesia lampas Dalz & Gibs. Ascorbic acid, gallic acid and indomethacin were used as reference standards for DPPH, ABTS and anti-lipox...