The detection of autoantibodies to epidermal or basement membrane zone proteins by immunoblot (IB) is useful for the diagnosis and the classification of autoimmune bullous diseases (AIBD). IB using human skin extracts is actually the reference method but A431 cell line is proposed as easier alternate antigen source. We explored the performances of “A431 IB” in comparison with the reference tech...

Monoclonal antibodies (MoAbs) were raised against epidermal growth factor (EGF) receptors on a human epidermoid carcinoma cell line, A431. Administration of anti-EGF receptor MoAbs inhib ited tumor formation in athymic mice by A431 cells and by another epidermal carcinoma cell line, 1222. When one of the same MoAbs was used in therapy against Li-7 (a human hepatoma) and HeLa cells (a cervical c...

aquatic plants have been used prevalently in china since 3000 years ago due to having various chemical compounds for diseases prevention and cure. dunaliella salina is one of the micro algae in marine ecosystems containing beta-carotene, retinal, apocarotenoides, ketones, aldehydes and epoxides which enable it to absorb free radicals and produce singlet oxygen.  in many studies, the anti-cancer...

Roles of cell adhesion molecules mediating the interaction of cancer and endothelial cells in tumor angiogenesis were investigated using new in vitro and in vivo model systems with a cultured murine endothelial cell line (F-2) and human cultured epidermoid cancer cells (A431). The A431 cells exhibited typical in vitro cell adhesion to the endothelial F-2 cells. The initial step of adhesion was ...

The aim of the present study was to investigate the effect of resveratrol on cell apoptosis, ability of telomerase and the human telomerase reverse transcriptase (hTERT) protein expression in human A431 epidermoid carcinoma cells. A431 cells were treated with different concentrations of resveratrol, and the cell appearance was then observed under a microscope. In addition, the cell proliferatio...

BACKGROUND Guibi-tang (GBT), a traditional herbal formula, mainly has been shown to possess immune regulation, antioxidant and protective effect of the gastric mucosa. Constituent herbs of GBT are frequently used to treat various diseases; however, their pharmacological effects, especially on cancer cells, differ from those of GBT. Furthermore, the molecular mechanisms behind effects of GBT rem...

A431 human epidermoid carcinoma cells monophenotypically express the placenta! alkaline phosphatase (PLAP)-like enzyme shown by its catalytic and antigenic characteristics, properties which are shared by the Nagao isozyme. More specifically, it is L-leucine sensitive just as is the rare placenta! D-variant of PLAP and the testicular heat-stable enzyme. Collectively, these are all referred to as...

The human epidermoid carcinoma cell line A431 has an usually high number of specific saturable receptor sites for the mitogenic polypeptide hormone epidermal growth factor (EGF). When A431 cells were treated with unlabeled EGF in the culture medium, approximately 50% of the EGF specific binding activity was lost within a few hours (receptor down regulation). A comparison of the surface proteins...

Human squamous carcinoma A431 cells express a high level of epidermal growth factor (EGF) receptor. The cells carry only a mutated form of the p53 gene, the G-->A mutation at codon 273 which results in an arginine to histidine substitution (mp53). The temporal changes of EGF receptor, c-Raf-1, mp53, and cell cycle distribution in A431 cells after 1-h exposure to doxorubicin (DOX) are examined. ...