Notes by Ana Kristine Torgerson" atorgers@u Case study, continued Sporulation summary What they did Measured mRNA expression levels of all 6200 yeast genes at 7 times points in a (loosely synchronized) sporulating yeast culture Plus some more standard tests and controls What they learned 3-10X increase in number of genes implicated in various subprocesses Several subsequently verified by direct...

Human SIRT1 is an enzyme that deacetylates the p53 tumor suppressor protein and has been suggested to modulate p53-dependent functions including DNA damage-induced cell death. In this report, we used EX-527, a novel, potent, and specific small-molecule inhibitor of SIRT1 catalytic activity to examine the role of SIRT1 in p53 acetylation and cell survival after DNA damage. Treatment with EX-527 ...

The notion of cofiber sequence is dual to that of fiber sequence. Most constructions and statements about fiber sequences readily dualize to cofiber sequences, though there are differences to be mindful of. 1. Fiber sequences were defined in the category of pointed spaces Top * , so that taking the strict fiber (i.e. preimage of the basepoint) makes sense. No such restriction exists for cofiber...

Sirtuins family are a Nicotinamide Adenine Dinucleotide (NAD+) dependent histone deacetylase enzyme. have been implicated in the pathogenesis of various diseases including cancer, neurological disorders and metabolic syndromes, hence sirtuins appointed as promising therapeutic target for diseases, by regulating its activity small molecules modulators. The indole containing selisistat (EX-527) d...

Alternate proof. Using a bit of differential topology (or a more geometric construction along the lines of Hatcher § 4.1 Exercise 15), consider the degree of a smooth map f : S → S. Since every homotopy class [f ] contains a smooth representative, and all such maps have the same degree (i.e. degree is a homotopy invariant), this defines a function deg : πn(S )→ Z. One readily shows that deg is ...