tomato is one of the most important vegetables in iran and throughout the world with crop loss caused by several viruses. this survey was carried out to detect the viruses causing mosaic symptoms on tomato in yazd city (iran). a total of 451 samples of tomato leaves showing mosaic symptoms were collected from fields and greenhouses during 2012-2013 growing season. collected samples were analyze...

In order to assess the occurrence, distribution and importance of viral diseases of mungbean and mashbean crops in Punjab, Pakistan, an extensive survey was conducted during summer season of 2004. The symptomatic plant samples were collected from farmer’s fields and experimental plots at research stations and tested by Enzyme-linked Immunosorbent Assay (ELISA) to detect and identify viruses inf...

The four alfalfa mosaic virus RNAs (respectively 24 S, 20 S, 17 S and 12 S) have been used separately as messengers in two in vitro protein synthesizing systems: wheat germ and rabbit reticulocyte lysate. In both systems a polypeptide corresponding to the translation of the entire length of the RNA can be found for RNAs 24 S, 20 S and 12 S, but not for 17 S RNA, the translation product of which...

Alfalfa mosaic virus genomic RNAs are infectious only when the viral coat protein binds to the RNA 3' termini. The crystal structure of an alfalfa mosaic virus RNA-peptide complex reveals that conserved AUGC repeats and Pro-Thr-x-Arg-Ser-x-x-Tyr coat protein amino acids cofold upon interacting. Alternating AUGC residues have opposite orientation, and they base pair in different adjacent duplexe...

Leaves of tobacco plant cv. Xanthi-nc inoculated or systemically infected with potato virus Y, cucumber mosaic virus, potato virus X, potato aucuba mosaic virus or alfalfa mosaic virus showed varying degrees of resistance to infection with tobacco mosaic virus. The resistance was correlated with the appearance of at least three proteins not present in healthy plants. These were the proteins tha...

Viruses are among the major agents causing yield loss in pepper production. During 2016-2017, a survey was performed pepperfields to determine of some viruses plants showing virus-like symptoms different provinces Turkey. Plant samples wereassayed using DAS-ELISA with specific antisera for cucumber mosaic virus (CMV), tomato spotted wilt (TSWV), yellow leafcurl (TYLCV), alfalfa (AMV), and mild ...

The present study was carried out to detect the presence of Alfalfa mosaic alfamovirus (AMV), cucumber mosaic cucumovirus (CMV), lettuce mosaic potyvirus (LMV), cucumber green mottle mosaic virus (CGMMV), tomato bushy stunt tombusvirus (TBSV), tobacco mosaic tobamovirus (TMV), tomato black ring nepovirus (TBRV) and tomato mosaic tobamovirus (ToMV) in seeds of pepper, tomato, cucumber and lettuc...

The three genomic RNAs of alfalfa mosaic virus each contain a unique 5' untranslated region (5' UTR). Replacement of the 5' UTR of RNA 1 by that of RNA 2 or 3 yielded infectious replicons. The sequence of a putative 5' stem-loop structure in RNA 1 was found to be required for negative-strand RNA synthesis. A similar putative 5' stem-loop structure is present in RNA 2 but not in RNA 3.

Promoter regions required for minus-strand and subgenomic RNA synthesis have been mapped for several plus-strand RNA viruses. In general, the two types of promoters do not share structural features even though they are recognized by the same viral polymerase. The minus-strand promoter of Alfalfa mosaic virus (AMV), a plant virus of the family Bromoviridae, consists of a triloop hairpin (hpE) wh...

In contrast to most alfalfa mosaic virus (AMV) strains (YSMV, S, M and 425), AMV strains VRU and 1 5/64 can form abnormally long virus particles, an ability which has been linked to the coat protein (CP). In order to study this phenomenon, the CP-encoding RNAs 3 of AMV strains VRU and 1 5/64 were cloned and fully sequenced. Comparative sequence analyses of AMV RNA 3 sequences derived from diffe...