Sovelli (1) described a color reaction for menadione, in which the compound was reacted with 2,4-dinitrophenylhydrazine, after which sodium hydroxide was added to alka1init.y. The green-colored product formed was extracted from the mixture with butyl alcohol. Attempts (24) have been made to apply this reaction quantitatively with variable results. Poor sensitivity, among ot)her things, was the ...

Anaemia constitutes a serious health problem in many tropical countries including Nigeria because of the high prevalence malaria and other parasitic infections which possibly leads to decrease hemoglobin. Fluted pumpkin has been reported be very good building constituents blood also replacing them. This study investigated effects methanolic leaves extract fluted on some hematological parameters...

A method is described for determining carbonyl groups that is especially suitable for use with proteins and peptides. It involves the determination of the extinction at 370nm of a sample solution after adding 2,4-dinitrophenylhydrazine. The reaction of 2,4-dinitrophenylhydrazine with pyruvoylglycine and with transaminated ribonuclease T(1) is presented; the isolation of protein hydrazones is di...

The determination of serum glutamic oxalacetic and glutamic pyruvic transaminase has been simplified by the use of automated analysis. The flow diagrams, concentration of reagents, and calculation of the color unit in terms of the Karmen unit are shown for 2,4-dinitrophenylhydrazine and 6-benzamido-4-methoxy-m -toluidine diazonium chloride as the reacting dyes. Advantages of both methods are co...

The X-ray structure of the 1:2 host-guest complex between 1,4,7,10,13,16-hexaoxacyclooctadecane (18-crown-6) and 2,4-dinitrophenylhydrazine is reported. Crystallography data are: space group P2i/c, a = 8.557(2), b = 16.745(6), c = 12.387(4) Ä, ß = 117.08(13)°, Z = 2, D m = 1.37 g • cm"3, D c = 1.387 g • cm-3 , m.p. 414 K (decomp.). The present B is 0.11 for 1733 diffractometer data. In the comp...

A comparison of two colorimetric procedures for the determination of serum glutamic oxaloacetic acid (SCOT) showed that both the dinitrophenylhydrazine and diazonium salt colorimetric assay procedures have good precision and reproducibility acceptable to most clinical laboratories. Babson’s diazonium salt procedure (3) definitely shows greater precision in the important borderline range of valu...