3T3 cells are shown to reduce SV40-3T3 cell population growth in a denisty-dependent manner in co-cultures of 3T3 and SV40-3T3 cells. The development of this inhibitory activity roughly parallels the development of density-dependent inhibition of growth in homogeneous 3T3 control cultures. The extent of reduction of SV40-3T3 growth can be manipulated by pretreatment of 3T3 cells with a high ser...

Marine Streptomyces are prolific producers of majority of bioactive secondary metabolites which are used in pharmaceutical industry as effective drugs against life threatening diseases. The cytotoxic activity of the pure compound 1, 2- benzene dicarboxylic acid, mono 2- ethylhexyl ester (DMEHE) from marine derived actinomycete Streptomyces sp. VITSJK8 was investigated against mouse embryonic fi...

background & aim: chorisia is an important bombacaceous plant that is traditionally used for a variety of ailments. due to its richness in several bioactive phytocompounds, some chorisia species showed a wide range of important biological effects. accordingly, the present work was undertaken to comparatively evaluate the antioxidant potential and effects on adipogenesis of chorisia chodatii...

A continuous cell line of highly contact-inhibited cells (NIH/3T3) has been developed from NIH Swiss mouse embryo cultures. Its growth properties are similar to those of 3T3 and BALB/3T3. Although 3T3 is relatively insensitive to focus formation by murine sarcoma viruses, cloned lines of both NIH/3T3 and BALB/3T3 have been isolated that are highly sensitive to sarcoma virus focus formation and ...

Balb/c 3T3 and simian virus-40-transformed Balb/c 3T3 (SV-3T3) cells were examined for their ability to transport nucleosides. Confluent (quiescent) 3T3 cells transported uridine at a rate 3-4 fold lower than did subconfluent cells. Adenosine uptake was independent of cell population density. Both adenosine and uridine were transported at the same rate by confluent and sub-confluent SV-3T3 cell...

The role of the NADPH oxidase homolog 1 (Nox1) in plasma membrane H(+) conductance and cellular H(+) production was investigated in 3T3 cells stably expressing Nox1 (Nox1 3T3) compared to vector-expressing control cells (mock 3T3). In whole cell patch clamp experiments both Nox1 and mock 3T3 expressed a similar H(+) conductance (Nox1 3T3, 13.2+/-8.6 pS/pF; mock 3T3, 16.6+/-13.4 pS/pF) with a nu...

Expression studies have consistently identified tumor protein D52 (TPD52) overexpression in tumor cells. Murine TPD52 (mD52) shares 86% identity with the human orthologue. To study a possible role for TPD52 in transformation, 3T3 fibroblasts were transfected with the full-length cDNA for mD52. Expression of mD52 was confirmed by reverse transcription-PCR (RT-PCR), real-time PCR, and Western blo...

The activity and turnover of dihydrolipoamide dehydrogenase (E3), the common component of the three 2-oxoacid dehydrogenase complexes, were measured during the differentiation of 3T3-L1 preadipocytes into 3T3-L1 adipocytes. The specific activity of E3 increased approx. 3-4-fold in 3T3-L1 adipocytes differentiated under a regimen of insulin, dexamethasone and 3-isobutyl-1-methylxanthine for 48 h...