A cluster of four different leghemoglobin (Lb) genes was isolated from AluI-HaeIII and EcoRI genomic libraries of soybean in a set of overlapping clones which together include 45 kilobases (kb) of contiguous DNA. These four genes, including a pseudogene, are present in the same orientation and are arranged in the order: 5'-Lba-Lbc1-Lb psi-Lbc3-3'. The intergenic regions average 2.5 kb. In addit...

the growth hormone receptor (ghr) is a member of cytokine/hematopoietin family that mediates the biological actions of growth hormone (gh) on target tissues. therefore, the purpose of this study was to examine the association of tg-repeat polymorphisms in the 5’-flanking region of bovine ghr gene with milk production traits and somatic cell score (scs) in holstein cattle of iran. the part of 5’...

Sowct: 1111tl DL'.I"Niplion of C/onl!: A 2 kb EmRI fr¡¡gnwut from thc 3' flankiug n:gion of thc hUillilll aortic smooth n1uscle actin gene thal Wits subdoncd inlu pUCI~ 1 {pASMDc).

lactoferrin (lf) is a bioactive protein present in all external secretions such as milk that plays important role in the innate host defense. therefore, the lf has a major role in bovine mammary gland defense during mastitis. in this study, 5’-flanking region of bovine lf gene containing three single nucleotide polymorphisms (snps) at positions −602 (t/g), −600 (a/g) and -586 (t/c) were screene...

HL-60 cells have an elevated level of cellular myc RNA due to an amplified c-myc gene. Subsequent to chemically induced differentiation of HL-60 cells, both cellular myc RNA levels (Grosso, L. E., and Pitot, H. C., Biochem. Biophys. Res. Commun., 119: 473, 1984) and myc-specific transcription decrease (Grosso, L. E., and Pitot, H. C., Cancer Res., 45: 847-850, 1985). We have compared the primar...

The evolutionary history of a region of DNA encompassing the Adh locus is studied by comparing patterns of variation in Drosophila melanogaster and its sibling species, D. simulans. An unexpectedly high level of silent polymorphism in the Adh coding region relative to the 5' and 3' flanking regions in D. melanogaster is revealed by a populational survey of restriction polymorphism using a four-...

Transcriptional fusions composed of a 19 kilodalton zein cDNA, the 5' flanking region from a beta-phaseolin gene, and 3' flanking regions from either the phaseolin or a 15-kilodalton zein gene were introduced into Petunia by Agrobacterium-mediated transformation. The expression of both zein mRNA and protein in these transgenic plants was seed-specific and developmentally regulated. Both monocot...

Not all members of the human histone gene family are functional. We have isolated a human H2B pseudogene that contains alterations in the protein-coding sequences as well as in the 3' and 5' flanking sequences that preclude expression of a functional H2B histone protein. There are three modifications in the amino acid-coding region: a single-base deletion producing a frame shift, a single-base ...

The human cathepsin G (CG) gene is expressed only in promyelocytes and encodes a neutral serine protease that is packaged in the azurophil (primary) granules of myeloid cells. To define the cis-acting DNA elements that are responsible for promyelocyte-specific "targeting," we injected a 6-kb transgene containing the entire human CG gene, including coding sequences contained in a 2.7-kb region, ...