MATERIALS AND METHODS Vinblastine was incubated in 0.2 M glycine buffer (pH 7.4 or 8.8) containing bovine serum albumin (1%) at 37°Cfor 72 h. The reaction mixture was extracted with CH2CI2, and the extract gave 6 major peaks (A, B, C, D, E, and F) with retention times of 5.0, 7.5, 11.0, 13.0, 23.0, and 30.0 min, respectively, in a high-performance liquid chromatography system (/¿Bondapak CÃ-a...

Hidden Markov Models need a large set of parameters which are induced from a text-corpus. The parameters should be optimal in the sense that resulting models assign high probabilities to seen training data. There are several methods to estimate model parameters. The first one is to use each word as a state and estimate the probabilities using the relative frequencies. The second method is a var...

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The aim of this work was to establish a temperature finish rolling stage Nb/Ti microalloyed steel containing 0.06 wt.% C, 0.77 Mn, 0.039 Nb and 0.015 Ti, using physical simulation. Samples were subjected laboratory simulation at twist plastometer high temperatures, i.e. between 825 950?C. Five pass deformation interpass times selected in accordance with processing parameters five stand finishin...

Vinblastine was incubated in 0.2 M glycine buffer (pH 7.4 or 8.8) containing bovine serum albumin (1%) at 37 degrees C for 72 h. The reaction mixture was extracted with CH2Cl2, and the extract gave 6 major peaks (A, B, C, D, E, and F) with retention times of 5.0, 7.5, 11.0, 13.0, 23.0, and 30.0 min, respectively, in a high-performance liquid chromatography system (muBondapak C18 column; solvent...