Introduction: The antibodies have an important role in the serodiagnosis, constituting the most widely used biomarkers to detect and confirm various diseases. Objective: To investigate the reproducibility of anti-human immunodeficiency virus (HIV) antibodies reactivity, to assess the stability of the sera samples stored at -20oC for two to eighteen years. Method: Sera were collected in the peri...

In the present study, the potential mutagenic effects of a packaging system for food containing oxygen absorbers was evaluated on a popular sweet bakery product (glazed doughnuts) stored at 20oC along its extended self life by using the Salmonella typhimurium Histest with the tester strains TA98, TA100, TA1535 and TA1537 and Escherichia coli Trptest with strains WP2uvrA and WP2uvrA pKM101. All ...

Blood samples are extensively used for PCR-based diagnosis of microbial infections and screening for blood banking1. It is not possible to do PCR with fresh blood samples each time. This preliminary study is aimed to compare the effect of blood storage (–20oC) on PCR for detecting Plasmodium falciparum in the two most commonly used anticoagulants such as ethylenediaminetetra acetic acid (EDTA) ...

This study addresses a matter of importance for: healthy infants; sick infants in the Neonatal Intensive Care Units; infants fed expressed human milk and infants who receive milk from Human Milk Banks. Current storage parameters for freezing of mother’s milk are not well established and are often contradictory. Pooled fresh human milk was stored raw, in nitrogen gas and following Holder pasteur...

Purified epsilon prototoxin of Clostridium perfringens type D was produced, purified, and detoxified by the stoiechiometric method of non-radioactive iodine incorporation. Different degrees of iodination were perfomed and the toxicity of the derivatives were analysed by in vivo studies. Toxicity decreased inversely to the iodine incorporation. Eletrophoretic analysis showed different levels of ...

The development of rancidity and its effect on quality loss was studied in frozen horse mackerel (Trachurus trachurus). For that, two different kinds of fish products (whole fish and fillets) were stored at a commercial frozen temperature (-20oC) for up to 12 months and were compared to samples stored at a much low temperature (-80oC). Analyses included: lipid hydrolysis (free fatty acid format...

Dye decolorizing bacteria was isolated and identified from textile effluent samples. The isolate was identified as Bacillus licheniformis by 16S rDNA sequencing. Azoreductase, an enzyme responsible for catalyzing the reductive cleavage of azo bonds was purified from B.licheniformis. The enzyme was purified by a combination of ammonium sulphate precipitation, ion exchange and affinity chromatogr...

Bandgap Reference voltage chip is implemented in 0.25μm CMOS technology with ESD protection. This chip can be designed by using a layout tool micro wind 3.1.7 version. The chip circuit generates a reference voltage of 1.23 V. It can operate between 20oC & 70oC temperature. Band gap core produces a voltage that is insensitive to variation in temperature. It has a unique protection with respect t...

The frequency independent critical concentration (Cc) of xanthan and carob (X/C) mixed gel was determined based on the Winter-Chambon’s theory. X/C mixed (X/C=1:1 ratio) gels were prepared from 0.1 to 1% of concentration. The linear viscoelastic properties, i.e., storage and loss modulus, of X/C mixed gel at 20oC were measured by frequency sweep tests. The frequency independence of tangent func...