objective: the development of combining mesenchymal stem cells (mscs) with surface modified three-dimensional (3d) biomaterial scaffold provides a desirable alternative for replacement of damaged and diseased tissue. nanofibrous scaffolds serve as suitable environment for cell attachment and proliferation due to their similarity to the physical dimension of the natural extracellular matrix. in ...

background: endometriosis is the presence of endometrial glandular and stromal cells outside of the uterine cavity. our previous study showed that in vitro culture of human endometrial tissue in a three-dimensional (3d) fibrin matrix could mimic the early stages of endometriosis with invasion gland and stroma formation and sprouting of new vessels. objective: the objective of the present study ...

background: while articular chondrocytes are among those appropriate candidates for cartilage regeneration, the cell dedifferentiation during monolayer culture has limited their application. several investigations have indicated the usefulness of alginate, but the topic of proliferation and differentiation of chondrocytes in alginate culture has still remained controversial. methods: rat articu...

allogeneic hematopoietic stem cell transplantation is used in the treatment of patients suffering from hematologic and non-hematologic disorders, but the application is limited by the identification of a suitable donor. umbilical cord blood (ucb) is an alternative source of hematopoietic stem cell (hsc) transplantation. despite all advantages, the limited cell dose is one of the  major obstacle...

cancer cell-stromal cell interaction plays a crucial role in the growth and invasion of tumor cells of various organs. it has been recently suggested that adipocytes and preadipocytes, two specific types of stromal cells, affect the biological behavior of variety of breast cancer cell lines. in spite of few investigations, there is a controversy in the literatures about the effects of adipocyte...

objective: spermatogonial stem cells (sscs) are the only cell type that can restore fertility to an infertile recipient following transplantation. much effort has been made to develop a protocol for differentiating isolated sscs in vitro. recently, three-dimensional (3d) culture system has been introduced as an appropriate microenvironment for clonal expansion and differentiation of sscs. this ...

abstract background: hyperthermia (also called thermal therapy or thermotherapy) is a type of cancer treatment in which body tissue is exposed to high temperatures (up to 113°f). research has shown that high temperatures can damage and kill cancer cells, usually with minimal injury to normal tissues.  for many years, biologists have investigated cancer by using monolayer cell culture. it is bec...

background: human umbilical cord blood (hucb) is an acceptable and readily accessible source of stem cells. there is an ongoing interest in cord blood stem cell therapies; however, little is known about the possible unfavorable effects of laboratory modifications on the isolated hucb cells. the involvement of mirnas in several biological processes has been shown. the aim of this study was to ev...

Alginate, a non-toxic polysaccharide isolated from brown algae, is a widely used 3-dimensional (3D) porous scaffold for the granulosa cell and follicle encapsulation. However, impurities in commercial alginate can lead to alginate biocompatibility reduction. The aim of this study was to evaluate in vitro behavior of the granulosa cells seeded on the purified alginate in varying concent...