Background: It was reported in a literature that approximately one third of the 4-cell stage embryos did not exhibit a tetrahedral shape. Non-tetrahedral embryos showed a lower in vitro developmental potential than tetrahedral embryos. Recently vitrification technology has been widely employed for embryo cryopreservation. The objective of this study was to prove our hypothesis that vitrified - ...

  SUMMARY To study the effect of vero cells on preimplantation mouse embryos two groups of experiment were carried out. In experiment 1, pronuclear stage mouse embryos were co-cultured with Vero cells in RPMI medium and were examined under microscope for 4 days with 24h interval. The results showed co-cultured group developed better compared to control (87% to 80%, P<0.05) although, the rate ...

Background: The aim of the study was to compare the effects of two different concentrations of cryoprotectants by Cryotop vitrification on survival and Heat shock protein 72 (Hspa1a) expression of two-cell mouse embryos. Materials and Methods: Different cryoprotectants’ concentrations of the combination of dimethyl sulfoxide (DMSO) and ethylene glycol (EG) were used and compared with each other...

background: the mitochondria are an important source of adenosine triphosphate (atp) production in pre-implantation embryo. therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and atp content. methods: the embryos at 2-pn, 4-cell and blastocyst stages were collected from the oviduct of st...

introduction: the mouse embryos can successfully be vitrified using ethylene glycol as cryoprotectant, however their development differs significantly from the non-vitrified embryos. the ability of their development can be improved when they co-culture with somatic cells. in the present study, the effects of co-culturing with vero cells on the development of vitrified two cell mouse embryos wer...

Objective A unique feature of embryo metabolism is production of reactive oxygen species (ROS). It is well established that during in vitro culture, ROS levels increase over normal ranges observed for embryos developed in vivo. This study evaluates and compares the stepwise pattern of ROS production during in vitro development of reconstructed goat embryos produced by zona-free method of somati...

Background Timing of the first zygotic cleavage is an accurate predictor of embryo quality. Embryos that cleaved early (EC) have been shown to exhibit higher developmental viability compared to those that cleaved at a later period (LC). However, the viability of EC embryos in comparison to LC embryos after vitrification is unknown. The present study aims to investigate the post-vitrification de...