A facile and novel synthesis of thirteen 2-amino-3-cyanopyridine derivatives 5(a–m) by a one-pot multicomponent reactions (MCRs) is described for the first time, starting from aromatic aldehydes, malononitrile, methyl ketones, or cyclohexanone ammonium acetate in presence nanostructured diphosphate Na2CaP2O7 (DIPH) at 80 °C under solvent-free conditions. These compounds were brought into existe...

The nitrilase which occurs abundantly in cells of Rhodococcus rhodochrous J1 catalyzes the direct hydrolysis of 3-cyanopyridine to nicotinic acid without forming nicotinamide. By using resting cells, the reaction conditions for nicotinic acid production were optimized. Under the optimum conditions, 100% of the added 3-cyanopyridine could be converted to nicotinic acid, the highest yield achieve...

The ;black yeast' Exophiala oligosperma R1 can utilise various organic nitriles under acidic conditions as nitrogen sources. The induction of a phenylacetonitrile converting activity was optimised by growing the strain in the presence of different nitriles and /or complex or inorganic nitrogen sources. The highest nitrile hydrolysing activity was observed with cells grown with 2-cyanopyridine a...

Abstract The reaction of Mn(NCS) 2 with 3‐cyanopyridine in water accidentally leads to the formation single crystals (H O) ( 1 ). If synthesis is performed without 3‐cyanopyridine, known tetrahydrate 4 formed. In crystal structure new hydrate, Mn 2+ cations are linked by pairs μ‐1,3‐bridging anionic ligands chains, which further connected via units into layers, that additionally stabilized intr...

Nitrilase from Rhodococcus rhodochrous ATCC 33278 hydrolyses both aliphatic and aromatic nitriles. Replacing Tyr-142 in the wild-type enzyme with the aromatic amino acid phenylalanine did not alter specificity for either substrate. However, the mutants containing non-polar aliphatic amino acids (alanine, valine and leucine) at position 142 were specific only for aromatic substrates such as benz...

Abstract Cyanopyridylalanines are non‐canonical amino acids that react with aminothiol compounds under physiological conditions in a biocompatible manner without requiring added catalyst. Here we present newly developed aminoacyl‐tRNA synthetases for genetic encoding of meta ‐ and para ‐cyanopyridylalanine to enable the site‐specific attachment wide range different functionalities. The outstand...

Nicotinic acid is widely used in the food, pharmaceutical and biochemical industries. Compared to chemical methods, enzymatic conversion of 3-cyanopyridine is an advantageous alternative for the production of nicotinic acid and nicotinamide. The separation of the product is complicated, owing to its high dilution rate in fermentation broth and high cost. Reactive liquid-liquid extraction by a s...