Tourniquet application is a widely accepted adjuvant technique in extremity surgery. The purpose of this prospective, randomized trial was to evaluate the effect of cuff width on skeletal muscle ischemia-reperfusion injury. A 2- or 4-cm wide curved tourniquet cuff was applied around the midthigh of 36 New Zealand White rabbits and inflated to a pressure of 200 or 400 mm Hg for 2 hours: group A=...

PCR Conditions: PCR was performed in a total volume of 20 microliters with 200 ng of genomic DNA, 8 pmoles of each primer, 2 mM MgCl2, 200 mM dNTP, 50 mM KC1, 5 mM Tris-HCl, pH 8.3, 0.01% gelatin, 0.5 U Taq polymerase (Boehringer). Primer Ang5 was 5' end labeled with P 7-ATP with the resulting specific activity 0.1-0.05 /iCi/pmol. Amplification was for 35 cycles with denaturation at 94°C 1 min,...

Salt stress is known to reduce root hydraulic conductivity and growth. To examine a concomitant regulation of aquaporins, the expression of the maize MIP gene family in response to NaCl was analysed by DNA array hybridization. Plants responded differentially to 100 versus 200 mM NaCl treatments. Leaf water content was reduced rapidly and persistently after the application of 200 mM NaCl in cont...