The dopamine receptor subtype in brain is DRD1. This receptor modulates several cellular functions and its expression has been studied in detail concerning extracellular signaling. A publication [1] indicates that there is also miRNA regulation (suppression) of DRD1 transcription and translation due to miR-142-3p interaction with the DRD1 3'-untranslated region (1,277 bp) of the DRD1 mRNA. This...

BACKGROUND Detection of Plasmodium vivax specific antibodies with serological tests could be a valuable tool for epidemiological researches. Whereas P. vivax cannot be simply obtained in vitro, serological tests using total or semi-purified antigens are infrequently used. Given this restriction, the present study investigated whether recombinant P. vivax merozoite surface protein 1 (PvMSP-1 42 ...

background: haemonchosis has a negative effect on the farming industry throughout the world, especially in the tropic and sub-tropic countries. the present study was carried out to differentiate haemonchus species from its main hosts in iran, including sheep, goat and camel.   methods: the identification took place based on the morphometrics of the spic-ules and molecular characters. two hundre...

A protein with a molecular mass of 19 kDa was isolated and purified from enriched membrane fractions of the virulent Erdman strain of Mycobacterium tuberculosis. The protein is different from another 19-kDa protein, a lipoprotein, that was recently described (D. B. Young and T. R. Garbe, Res. Microbiol. 142:55-65, 1991). The sequencing strategy applied to this major membrane protein employed fo...

Clinical immunity to malaria declines in the absence of repeated parasite exposure. However, little is known about how B cell populations and antigen-specific memory B cells change in the absence of P. falciparum infection. A successful indoor residual insecticide spraying campaign in a highland area of western Kenya, led to an absence of blood-stage P. falciparum infection between March 2007 a...

A nucleosome assembly protein (AP-I) was purified approximately 50% from the cytosol of HeLa S3 cells by three purification steps. Using this protein fraction as an antigen, we established three stable hybridomas that secrete monoclonal antibodies specific for AP-I by the conventional method of cell fusion. Immunoblotting of the HeLa S3 cytosol, proved AP-I exists as a 58-kDa peptide in vivo, n...

A 426 bp cDNA encoding a predicted small heat shock protein (smHSP) was isolated from Wuchereria bancrofti lambda zap L3 cDNA expression library by immunoscreening with microfilaremic sera. The open reading frame of the cDNA clone encodes a predicted protein of 142 amino acids (aa), which had high sequence identity with other nematode smHSPs. The homologous regions conserved in several differen...

Human adenosine deaminase (ADA) occurs as a 41-kDa soluble monomer in all cells. On epithelia and lymphoid cells of humans, but not mice, ADA also occurs bound to the membrane glycoprotein CD26/dipeptidyl peptidase IV. This "ecto-ADA" has been postulated to regulate extracellular Ado levels, and also the function of CD26 as a co-stimulator of activated T cells. The CD26-binding site of human AD...

We isolated cDNA clones coding for the functionally important tryptic N-terminal 38-kDa fragment of human complement control protein factor H using polyclonal and monoclonal antibodies to screen a human liver cDNA library cloned in a bacterial expression vector, PEX-1. By testing the reactivity of antibodies specific for the recombinant proteins produced by individual clones with proteolytic fr...