BACKGROUND AND OBJECTIVES DNA molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. MATERIALS AND METHODS Here a PCR-supported procedure is described that uses 10 primer pairs targeting chromosomal DNA from the harmless vaccinal Bacillus anthracis Sterne 34F2 strain as template. A single PCR protocol is used to reproduce a...

To detect the genome of viruses (in environmental and clinical samples), we use electrophoresis running buffer after PCR reaction. Also, buffers were used widely to separate any DNA molecule. In this paper, four types previously known compare which is easy for preparation, simple in structure, low cost good performance agarose gel electrophoresis. For this, two concentration (1%, 2%) ladder (10...

We evaluated a novel strategy for fast diagnosis by microchip electrophoresis (ME), using programmed field strength gradients (PFSG) in a conventional glass double-T microfluidic chip. The ME-PFSG allows for the ultrafast separation and enhanced resolving power for target DNA fragments. These results are based on electric field strength gradients (FSG) that use an ME separation step in a sievin...

Active development of compact analytical instruments suitable for point-of-care testing (POCT) requires optimization of existing methods. To aid the development of capillary gel electrophoresis instruments for POCT, we attempted to separate polymerase chain reaction products (small DNAs) using a short, fused silica capillary coated with an acrylamide (AM)/acrylic acid (AA) copolymer (poly(AM-co...

TG572 RFLP Probe: Five primers were designed around the RFLP probe on chromosome 7: P572F1, P572F2, P572F3, P572R1, and P572R2 (Table 1). All possible primer combinations produced a strong band with Heinz 1706 DNA between 600 bp and 1000 bp (Fig. 2). All of the primer combinations produced single bands with the exception of P572F2/P572R1, which produced two bands one at 400 bp and one at 900 bp...

Fig. S1. Results of polymerase chain reaction (PCR) using universal primers targeting mycobacterium DNA: hsp65 short (hsp65S; 441 bp), long (hsp65L; ca.770 bp), rpoB short (rpoBS; ca.330bp), long (rpoBL; ca.450bp) genes and the 16S-23S spacer region (internal transcribed spacer (ITS); ca. 350 bp). Clear strong bands with hsp65L, rpoBS, and ITS primers proved the existence of mycobacteria DNA. I...

Two new primer sets of a 766- and a 344-bp fragment were introduced into the conventional Bruce-ladder PCR assay. This novel multiplex PCR assay rapidly and concisely discriminates Brucella canis and Brucella microti from Brucella suis strains and also may differentiate all of the 10 Brucella species.

1This project is supported by grants from the National Natural Science Foundation of China; Ministry of Agriculture, China, and Sino-German Agricultural Cooperation Program. 2Present address: Centre for Genetic Improvement of Livestock, Dept. of Anim. and Poult. Sci., Univ. of Guelph, Guelph, Ontario N1G 2W1 Canada. 3To whom correspondence should be addressed (phone: 08161 71 3746; E-mail: rott...

Sequencing of DNA fragments of 130 and 200 bp using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for DNA ladder detection was demonstrated. With further improvement in mass resolution and detection sensitivity, mass spectrometry shows great promise for routine DNA sequencing in the future.

with the thymine residues were isolated and ligated together in a 10-μL reaction mixture (overnight at room temperature) using equal amounts of each fragment. The ligation was terminated by heating to 65°C, and the products were introduced into DH5α E. coli cells. Cells were grown under ampicillin selection and white colonies selected for miniprep isolation. Plasmid DNA was digested with PvuII...