RNA-guided nucleotide modification complexes direct the post-transcriptional nucleotide modification of both archaeal and eukaryotic RNAs. We have previously demonstrated that efficient 2'-O-methylation activity guided by an in vitro reconstituted archaeal box C/D sRNP requires juxtaposed box C/D and C'/D' RNP complexes. In these experiments, we investigate the importance of spatially positioni...

Archaeal box C/D RNAs guide the site-specific 2'-O-methylation of target nucleotides in ribosomal RNAs and tRNAs. In vitro reconstitution of catalytically active box C/D RNPs by use of in vitro transcribed box C/D RNAs and recombinant core proteins provides model complexes for the study of box C/D RNP assembly, structure, and function. Described here are protocols for assembly of the archaeal b...

It is known that a PR-BOX (PR), a non-local resource and (2 → 1) random access code (RAC), a functionality (wherein Alice encodes 2 bits into 1 bit message and Bob learns one of randomly chosen Alice’s inputs) are equivalent under the no-signaling condition. In this work we introduce generalizations to PR and (2→ 1) RAC and study their inter-convertibility. We introduce generalizations based on...

The problem of finding the largest empty axis-parallel box amidst a point configuration is a classical problem in computational complexity theory. It is known that the volume of the largest empty box is of asymptotic order 1/n for n → ∞ and fixed dimension d. However, it is natural to assume that the volume of the largest empty box increases as d gets larger. In the present paper we prove that ...

We present a novel method for the quantification of transport, chemistry, and mixing along atmospheric trajectories based on a consistent model hierarchy. The hierarchy consists of the new atmospheric-chemistry trajectory-box model CAABA/MJT and the three-dimensional (3-D) global ECHAM/MESSy atmospheric-chemistry (EMAC) general circulation model. CAABA/MJT employs the atmospheric box model CAAB...

We consider the use of bounding boxes to detect collisions among a set of convex objects in R d. We derive tight bounds on the ratio between the number of box intersections and the number of object intersections. Connrming intuition, we show that the performance of bounding boxes improves signiicantly when the underlying objects are all convex. In particular, the ratio is (1?1=d 1=2 box) if eac...

There are two main classes of small nucleolar RNAs (snoRNAs): the box C/D snoRNAs and the box H/ACA snoRNAs that function as guide RNAs to direct sequence-specific modification of rRNA precursors and other nucleolar RNA targets. A previous computational and biochemical analysis revealed a possible evolutionary relationship between miRNA precursors and some box H/ACA snoRNAs. Here, we investigat...