A newly introduced sulphydryl compound, dithiothreitol (DTT), is evaluated for its optimal conditions of inactivation of IgM antibodies. The maximal effects of DTT reagent are observed when its final concentrations are between 00025 M and 0005 M, pH between 7 0 and 8O0, and incubation at 370C. Concentrations over 0 01 M, pH values greater than 8-0, and an incubation temperature over 40'C result...