The presence of numerous G protein-coupled receptor (GPCR) homologs within the herpesvirus genomes suggests an essential role for these genes in viral replication in the infected host. Such is the case for murine cytomegalovirus (MCMV), where deletion of the M33 GPCR or replacement of M33 with a signaling defective mutant has been shown to severely attenuate replication in vivo. In the present ...

The branched M33 antimicrobial peptide was previously shown to be very active against Gram-negative bacterial pathogens, including multidrug-resistant strains. In an attempt to produce back-up molecules, we synthesized an M33 peptide isomer consisting of D-aminoacids (M33-D). This isomeric version showed 4 to 16-fold higher activity against Gram-positive pathogens, including Staphylococcus aure...

The Drosophila gene Polycomb (Pc) has been implicated in the clonal inheritance of determined states and is a fra/is-regulator of the Antennapedia-like homeobox genes. Pc shares a region of homology (the chromobox) with the Drosophila gene Heterochromatin Protein 1 (HP1), a component of heterochromatin. The Pc chromobox has been used to isolate a mouse chromobox gene, M33, which encodes a predi...

Mice with disrupted mammalian PcG (Polycomb group) genes commonly show skeletal transformation of anterior-posterior identities. Disruption of the murine M33 gene, a PcG member, displayed posterior transformation of the vertebral columns and sternal ribs. In addition, failure of T-cell expansion and hypoplasia and sex-reversal of the gonads, have been observed. In the present study, we identifi...

The nearby galaxy M33 was observed by the imaging X-ray instruments on-board BeppoSAX. Two observations at di erent phases of the 105.9 day intensity cycle of the luminous central source X-8 failed to reveal the expected modulation, suggesting that it is probably transitory. Similar behavior has been observed from several X-ray binary sources. This strengthens somewhat the idea that M33 X-8 is ...

The nearby galaxy M33 was observed by the imaging X-ray instruments on-board BeppoSAX. Two observations at different phases of the 105.9 day intensity cycle of the luminous central source X-8 failed to reveal the expected modulation, suggesting that it is probably transitory. Similar behavior has been observed from several X-ray binary sources. This strengthens somewhat the idea that M33 X-8 is...

Mice with disrupted mammalian PcG (Polycomb group) genes commonly show skeletal transformation of anterior-posterior identities. Disruption of the murine M33 gene, a PcG member, displayed posterior transformation of the vertebral columns and sternal ribs. In addition, failure of T-cell expansion and hypoplasia and sex-reversal of the gonads, have been observed. In the present study, we identifi...

We have determined the distance to M33 using single epoch I-band observations of Cepheids based on HST/WFPC2 images of five fields in M33. Combining the HST I-band photometry and the periods determined from the ground-based observations (DIRECT) for 21 Cepheids with logP > 0.8 in the sample of 32 Cepheids, we derive a distance modulus of (m−M)0 = 24.52±0.14(random)±0.13(systematic) for an adopt...

M33, encoded by murine cytomegalovirus (MCMV), is a member of the UL33 homolog G-protein-coupled receptor (GPCR) family and is conserved across all the betaherpesviruses. Infection of mice with recombinant viruses lacking M33 or containing specific signaling domain mutations in M33 results in significantly diminished MCMV infection of the salivary glands. To determine the role of M33 in viral d...