Viral protein 2 (VP2) of canine parvovirus (CPV) exhibits a high degree genetic and antigenic diversity. We analyzed 88 Vietnamese CPV-VP2 sequences (1755 bp), 34 from this study 54 previous studies, discovered new sublineage, “new var.”, within the lineage CPV-2c-“new”, characterized by mutation 5G/447M, which is restricted to isolates. These mutants appear have emerged in recent years, accoun...

vp2 gene coding region of a vaccinal strain (d78) of infectious bursal disease virus (ibdv) was clonedin a eukaryotic expression vector, psec tag2a. the gene was placed downstream of ig κ chain leadersequence, under the control of human cytomegalovirus (hcmv) immediate early enhancer and promoter. theconstruct psec tag2a-vp2 was transfected in cos-7 cell line and the expression and secretion of...

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...

We investigated the roles of simian virus 40 capsid proteins in the viral life cycle by analyzing point mutants in Vp1 and Vp2/3, as well as a deletion mutant lacking the Vp2/3 coding sequence. The Vp1 mutants (V243E and L245E) and the Vp2/3 mutants (F157E-I158E and P164R-G165E-G166R) were previously shown to be defective in Vp1-Vp2/3 interaction and to be noninfectious or poorly infectious, re...

This paper describes the expression of a cloned African horsesickness virus (AHSV) serotype 5 VP2-gene by a baculovirus recombinant that was generated by the BAC-TO-BAC system. Immunization of horses with crude cell lysates containing recombinant baculovirus-expressed AHSV5 VP2 did induce neutralizing antibodies, but afforded only partial protection against virulent virus challenge. Further ana...

Rotaviruses are triple-layered particles that contain four major capsid proteins, VP2, VP4, VP6, and VP7, and two minor proteins, VP1 and VP3. We have cloned each of the rotavirus genes coding for a major capsid protein into the baculovirus expression system and expressed each protein in insect cells. Coexpression of different combinations of the rotavirus major structural proteins resulted in ...

BACKGROUND VP2 of chicken anemia virus (CAV) is a dual-specificity phosphatase required for virus infection, assembly and replication. The functions of the nuclear localization signal (NLS) and nuclear export signal (NES) of VP2 in the cell, however, are poorly understood. Our study identified the presence of a NLS in VP2 and showed that the protein interacted significantly with mini-chromosome...

BACKGROUND Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. Generally, CAV infection occurs via vertical transmission in young chicks that are less than two weeks old, which are very susceptible to the disease. Therefore, epidemiological investigations of CAV infection and/or the evaluation of the immunizati...

Background: The importance of viral protein-2 (VP2) of canine parvovirus (CPV) in binding to human cancer cells, production of veterinary vaccines and diagnostic kits has motivated several researches on producing this protein.Objectives: Our purpose was to construct recombinant bacmid shuttle vectors expressing VP2 of CPV using Bac-to-Bac baculoviral expr...

The development and use of recombinant vaccine vectors for the expression of poultry pathogens proteins is an active research field. The adeno-associated virus (AAV) is a replication-defective virus member of the family Parvoviridae that has been successfully used for gene delivery in humans and other species. In this experiment, an avian adeno-associated virus (AAAV) expressing the infectious ...