For most substrates of ubiquitin (Ub)-dependent degradation, recognition by the proteasome is mediated by a covalently attached signal assembled from multiple ubiquitins linked to each other via the C terminus of one Ub and the epsilon-amine of Lys(48) of another Ub. Among Ub-conjugating enzymes, E2-25K is unique in its ability to synthesize in vitro unanchored Lys(48)-linked poly-Ub chains fro...

In rings of rabbit facial vein (RFV), depletion of sarcoplasmic reticulum (SR) Ca2+ by caffeine abolished the subsequent isometric contraction to 25 mM K+ physiological salt solution (25K-PSS). However, the associated steady-state increase of smooth muscle intracellular free Ca2+concentration ([Ca2+]i), measured using fura PE3 and cuvette photometry, was not altered. Treatment with the specific...

Laporte, Régent, and Ismail Laher. Sarcoplasmic reticulum and endothelium independently regulate venous smooth muscle [Ca]i and contraction. Am. J. Physiol. 277 (Heart Circ. Physiol. 46): H749–H755, 1999.—In rings of rabbit facial vein (RFV), depletion of sarcoplasmic reticulum (SR) Ca21 by caffeine abolished the subsequent isometric contraction to 25 mM K1 physiological salt solution (25K-PSS)...

A series of polyethylenimines-coated poly(d,l-lactide-co-glycolide)/lipid nanoparticles (PPLs) were fabricated for delivering paclitaxel via a simple nano-precipitation method. Four kinds of polyethylenimines (PEIs) (800 Da-, 2000 Da- and 25 kDa-branched PEIs, and 25 kDa-linear PEI) were selected as a polymeric coating for the nanoparticles. The PPLs were evaluated for their cytotoxic effects t...

In experiments on mice the study of radioprotective and radiotherapeutic efficacy combined use selenium-containing feed additive DAFS-25k iodine-containing preparation Monclavit-1 was carried out. It has been shown that intragastric administration oil solution 3 hours before total, external, single exposure to γ-radiation, subsequent (in 12 hours) an aqueous Monclavit-1, increases survival rate...

The present study was carried out to determine the effects of fenugreek powder (FP) and extract (FE) on performance, egg quality, blood parameters and immune responses of laying hens. One-hundred and fifty Leghorn laying hens were used in a completely randomized design with five treatments and five replicates for eight weeks. Treatments were various levels of FP and FE including zero (control; ...

Aphis glycines Matsumura (Hemiptera: Aphididae) can reduce the yield of aphid-susceptible soybean (Glycine max (L.) Merrill) cultivars. The Rag1 and Rag2 genes conferresistance to some biotypes of A. glycines. These genes individually can limit population growth of A. glycines and prevent yield loss. The impact of these genes when combined is not known. We compared the development of A. glycine...