1. Thiobacillus strain C oxidized [35S]thiosulphate completely to sulphate. 2. During thiosulphate oxidation [35S]sulphate was formed more rapidly from (S. 35SO3)2than from (35S *SO3)2-. 35S disappeared less rapidly from thiosulphate with (35S SO3)2as substrate than with (S.35SO3)2-. 3. Thiosulphate labelled in both atoms was produced during (35S * SO3)2oxidation, but not during (S. 35SO3)2oxid...

In this study, we investigated the biochemical mechanisms of agonist action at the G protein-coupled D2 dopamine receptor expressed in Chinese hamster ovary cells. Stimulation of guanosine 5'-O-(3-[35S]thio)triphosphate ([35S]GTPgammaS) binding by full and partial agonists was determined at different concentrations of [35S]GTPgammaS (0.1 and 10 nM) and in the presence of different concentration...

Filter-grown Madin-Darby canine kidney (MDCK) cells labelled for 24 h with [35S]sulphate were found to secrete macromolecules [35S]sulphated on their carbohydrate moieties predominantly into the basolateral medium, whereas the tyrosine-[35S]sulphated proteins synthesized were predominantly secreted into the apical medium. In contrast with the predominant apical secretin of tyrosine-[35S]sulphat...

camv 35s promoter activity has not been analysed in different stages of growth in oil-seed (b. napus). higher plants lack intrinsic β-glucuronidase activity, thus enhancing the sensitivity with which measurements can be made. the binary transformation vector pvw432 (buchanan-wollaston, unpublished), carrying the gus gene fused to the camv 35s promoter, was used to test transformation efficienci...

[35S]Heparin was produced in vitro by incubation of rat peritoneal mast cells with [35S]sulfate and in vivo by injection of [35S]sulfate into rats. The [35S]heparin together with nonlabeled heparin in the mast cells was isolated in native form by mild methods that avoided the use of proteolytic enzymes or high alkali concentrations. The heparin had low anticoagulant activity. Incubations of mas...

Cosuppression results in the degradation of RNA from host genes and homologous transgenes after transcription in the nucleus. By using grafting experiments, we have shown previously that a systemic signal mediates the propagation of cosuppression of Nia host genes and 35S-Nia2 transgenes from silenced 35S-Nia2 transgenic stocks to nonsilenced 35S-Nia2 transgenic scions but not to wild-type scio...