نتایج جستجو برای: پروموتر patatin
تعداد نتایج: 743 فیلتر نتایج به سال:
The human genome expresses nine patatin-like phospholipase domain containing proteins (PNPLA1-9). Members of this family share a protein domain discovered initially in patatin, the most abundant protein of the potato tuber. Patatin is a lipid hydrolase with an unusual folding topology that differs from classical lipases. Mammalian PNPLAs include lipid hydrolases with specificities for diverse s...
To achieve the high level of gene expression, promoters are key elements. Patatin promoter has been used as a specific gene expression in potato tubers. Patatin is a group of glycol protein in potato tuber which is code by a multiple family genes. This protein makes up more than 40% of soluble proteins of potato tubers. Class I Promoters mainly responsible for tuber -specific expression patte...
تراریختی چغندر قند (Beta vulgaris L. ) با آگروباکتریوم ریزوژنز برای مطالعه بیان ژن در ریشه‌های مویین
در این تحقیق، با استفاده از آگروباکتریوم ریزوژنز، ریشه مویین چغندرقند تراریخته تولید گردید. در حین این مطالعه، تاثیر پروموتر جداسازی شده از آرابیدوپسیس تالیانا (پروموتر AT) که با پروموتر Hs1pro-1 ژن مقاومت به نماتد درچغندر مشابهت زیادی دارد، بر روی بیان ژن گزارشگر در مقایسه با پروموتر CaMV35S نیز مطالعه گردید. همچنین به عنوان کنترل منفی، ریشه‌های مویین با آگروباکتریوم ریزوژنز فاقد ناقل دوگا...
Adipose triglyceride lipase (ATGL) is the rate-limiting enzyme of lipolysis. ATGL specifically hydrolyzes triacylglycerols (TGs), thereby generating diacylglycerols and free fatty acids. ATGL's enzymatic activity is co-activated by the protein comparative gene identification-58 (CGI-58) and inhibited by the protein G0/G1 switch gene 2 (G0S2). The enzyme is predicted to act through a catalytic d...
The influence of N-glycosylation and subcellular compartmentation on various characteristics of a vacuolar glycoprotein is described. One member of the patatin gene family was investigated as a model system. Different glycosylation mutants obtained by destroying the consensus site Asn-X-Ser/Thr by oligonucleotide-directed mutagenesis were expressed in leaves of transgenic tobacco plants under t...
In transgenic Arabidopsis a patatin class I promoter from potato is regulated by sugars and proline (Pro), thus integrating signals derived from carbon and nitrogen metabolism. In both cases a signaling cascade involving protein phosphatases is involved in induction. Other endogenous genes are also regulated by both Pro and carbohydrates. Chalcone synthase (CHS) gene expression is induced by bo...
ExoU, a potent patatin-like phospholipase, causes rapid cell death following its injection into host cells by the Pseudomonas aeruginosa type III secretion system. To better define regions of ExoU required for cytotoxicity, transposon-based linker insertion mutagenesis followed by site-directed mutagenesis of individual residues was employed by using a Saccharomyces cerevisiae model system. Ran...
طی سی سال گذشته کلزا به یکی از محصولات عمده کشاورزی تبدیل شده است و در حال حاضر نیز سومین منبع روغن گیاهی پس از سویا و نخل روغنی می باشد. بذور آن علاوه بر سطح بالای لیپید، حاوی پروتئین قابل توجهی هستند که حدوداً 25-20 درصد از وزن خشک بذر را تشکیل می دهد. یکی از پروتئین های ذخیره ای مهم در جنین های کلزا پروتئین ناپین (napin) است که طی توسعه بذر ساخته می شود. سنتز پروتئین-های ذخیره ای در ابتدا در ...
The apicomplexan parasite Toxoplasma gondii is able to suppress nitric oxide production in activated macrophages. A screen of over 6000 T. gondii insertional mutants identified two clones, which were consistently unable to suppress nitric oxide production from activated macrophages. One strain, called 89B7, grew at the same rate as wild-type parasites in naïve macrophages, but unlike wild type,...
Tubers of a common tetraploid species of Solanum tuberosum (Granola) were mechanically wounded by slicing. After 18 hr only small differences were detectable in the pattern of the steady-state protein extracted from wounded versus unwounded tubers. In contrast the protein pattern obtained by in vitro translation of mRNA isolated from wounded and unwounded tubers differed significantly. A cDNA l...
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