BACKGROUND Therapeutic angiogenesis seeks to promote blood vessel growth to improve tissue perfusion. Vascular endothelial growth factor (VEGF) exists in multiple isoforms. We investigated an engineered zinc finger-containing transcription factor plasmid designed to activate the endogenous VEGF gene (ZFP-VEGF). METHODS AND RESULTS New Zealand White rabbits (n=56) underwent unilateral femoral ...

In the context of cell-free massive multi-input multi-output (mMIMO), zero-forcing precoding (ZFP) requires exchange instantaneous channel state information and precoded data symbols via a fronthaul network. It causes considerable propagation processing delays, which degrade performance. This letter analyzes impact aging on performance ZFP in mMIMO. The effects not only user mobility but also p...

Zinc finger proteins (Zfp) are members of a multigene family encoding Zn mediated nucleic acid binding proteins. They have been isolated from various organisms including yeast, Drosophila, Xenopus mouse and human. All Zfp share the 28-30 amino acid long finger repeats containing conserved residues at specific positions. Some of these proteins have been identified as transcriptional regulatory f...

BACKGROUND Artificial transcription factors (ATFs) are composed of DNA-binding and functional domains. These domains can be fused together to create proteins that can bind a chosen DNA sequence. To construct a valid ATF, it is necessary to design suitable DNA-binding and functional domains. The Cys2-His2 zinc finger motif is the ideal structural scaffold on which to construct a sequence-specifi...

The murine Zfp-37 gene encodes a protein with 12 zinc fingers at its C-terminus (Nelki et al., 1990, Nucleic Acids Res. 18: 3655; Burke and Wolgemuth, 1992, Nucleic Acids Res. 20: 2827-2834). Contrary to the published data, our Northern blot analysis demonstrates not only that the Zfp-37 gene is expressed as 2.3, 2.6, and 4.2 kb mRNAs in testis, but also that there is a 3.7-kb message in the ad...

The visual detection of specific double-stranded DNA sequences possesses great potential for the development of diagnostics. Zinc finger domains provide a powerful scaffold for creating custom DNA-binding proteins that recognize specific DNA sequences. We previously demonstrated sequence-enabled reassembly of TEM-1 β-lactamase (SEER-LAC), a system consisting of two inactive fragments of β-lacta...

Herein, we loaded cobalt-based zeolite imidazolate frameworks, ZIF-67 (Co), onto commercial filter paper to prepare catalytic (ZFP) for the in situ degradation of bisphenol A (BPA) water by activating peroxymonosulfate. The results showed that (Co) was densely and uniformly distributed on surface paper. prepared ZFP could effectively degrade BPA through a gravity-driven filtration process. Spec...

Insulin signaling has a profound effect on longevity and the oxidative stress resistance of animals. Inhibition of insulin signaling results in the activation of DAF-16/FOXO and SKN-1/Nrf transcription factors and increased animal fitness. By studying the biological functions of the endogenous RNA interference factor RDE-4 and conserved PHD zinc finger protein ZFP-1 (AF10), which regulate overl...

Insulin/IGF-1-like signalling (IIS) and dietary restriction (DR) are the two major modulatory pathways controlling longevity across species. Here, we show that both pathways license a common chromatin modifier, ZFP-1/AF10. The downstream transcription factors of the IIS and select DR pathways, DAF-16/FOXO or PHA-4/FOXA, respectively, both transcriptionally regulate the expression of zfp-1. ZFP-...