Prevention of virus infections is a major objective in agriculture and human health. One attractive approach to the prevention is inhibition of virus replication. To demonstrate this concept in vivo, an artificial zinc finger protein (AZP) targeting the replication origin of the Beet severe curly top virus (BSCTV), a model DNA virus, was created. In vitro DNA binding assays indicated that the A...

Pathogenic mycobacteria reside in, and are in turn controlled by, macrophages. However, emerging data suggest that neutrophils also play a critical role in innate immunity to tuberculosis, presumably by their different antibacterial granule proteins. In this study, we purified neutrophil azurophil and specific granules and systematically analyzed the antimycobacterial activity of some purified ...

This paper concerns the problem of matching incompatible zonal geographies, for example in the context of comparing census outputs over time. The automated zoning procedure (AZP) proposed by Openshaw (1977) is reviewed and extended to permit its application to the intersection of two zonal systems. A population stress statistic is proposed which may be used in the extended AZP algorithm in orde...

Spraydrift and edge-of-field runoff are important routes of pesticide entry into streams. Pesticide contamination originating from spraydrift usually resides in the water phase, while pesticides in contaminated runoff are to a large extent associated with suspended particles (SPs). The effects of two organophosphorous insecticides (OPs), chloropyrifos (CPF) and azinphos-methyl (AZP), on acetylc...

While unsupervised anaphoric zero pronoun (AZP) resolvers have recently been shown to rival their supervised counterparts in performance, it is relatively difficult to scale them up to reach the next level of performance due to the large amount of feature engineering efforts involved and their ineffectiveness in exploiting lexical features. To address these weaknesses, we propose a supervised a...

The signal recognition particle (SRP) from Escherichia coli consists of 4.5S RNA and protein Ffh. It is essential for targeting ribosomes that are translating integral membrane proteins to the translocation pore in the plasma membrane. Independently of Ffh, 4.5S RNA also interacts with elongation factor G (EF-G) and the 30S ribosomal subunit. Here we use a cross-linking approach to probe the co...

Genetically engineered cotton and corn plants producing insecticidal Bacillus thuringiensis (Bt) toxins kill some key insect pests. Yet, evolution of resistance by pests threatens long-term insect control by these transgenic Bt crops. We compared the genetic basis of resistance to Bt toxin Cry1Ac in two independently derived, laboratory-selected strains of a major cotton pest, the pink bollworm...

Cardiac microsomes were incubated with [y-azP]ATP and a cardiac adenosine 3’:5’-monophosphate (cyclic AMP)dependent protein kinase in the presence of ethylene glycol bis(&aminoethyl ether)-A’, IV’-tetraacetic acid. After solubilization in sodium dodecyl sulfate and fractionation by polyacrylamide gel electrophoresis, a single microsomal protein component of approximately 22,000 daltons was foun...

The present study evaluated the shear bond strength between an indirect composite material and zirconium dioxide (zirconia) ceramics after thermocycling. A total of 80 zirconia (Katana) discs were divided into five groups and primed with one of following agents: All Bond 2 Primer B (ABB), Alloy Primer (ALP), AZ Primer (AZP), Estenia Opaque Primer (EOP), and Porcelain Liner M Liquid A (PLA). An ...