Objective(s):Gentamicin sulphate (GS) nephrotoxicity seems to be related to the generation of reactive oxygen species. There is evidence that oxygen preconditioning increases the activity of antioxidant enzymes. Materials and Methods: Forty eight female rats were divided into 6 groups (n=8) as follows: group 1 was the control, group 2 received daily GS, groups 3,4 and 5 received oxygen 2 hr/day...

The GTPase activity of the stimulatory guanine nucleotide-binding regulatory protein (Gs) of hormone-sensitive adenylate cyclase was investigated using purified rabbit hepatic Gs and either [alpha-32P]- or [gamma-32P] GTP as substrate. The binding of [35S]guanosine 5'-O-(thiotriphosphate) (GTP gamma S) was used to quantitate the total concentration of Gs. 1) GTPase activity was a saturable func...

Introduction : The degree of differentiation prostate cancer is evaluated by the Gleason score (GS). Patients who undergo radical prostatectomy (RP) have their GS determined twice—first, during biopsy, and second, after RP. two GSs often differ. Aim: We studied how change affects biochemical progression-free survival (BPFS) patients. Materials Methods: patients were divided into three groups. G...

We report the discovery of the optical counterpart of the 12.3 s transient X–ray pulsar GS 0834–43. We re–analysed archival ROSAT PSPC observations of GS 0834–43 obtaining two new refined positions, ∼14 and ∼18 away from the previously published one, and a new spin period measurement. Based on these results we carried out optical and infra–red (IR) follow–up observations. Within the new error c...

PURPOSE We aimed to determine whether 12 core-extended biopsies of the prostate could predict insignificant prostate cancer (IPCa) in Koreans reliably enough to recommend active surveillance. MATERIALS AND METHODS Two hundred and ninety-seven patients who underwent radical prostatectomy after 12 core-extended prostate biopsies were retrospectively reviewed. 38 cases (12.8%) were shown to be I...

Glutamine synthetase (GS) utilizes various substituted glutamic acids as substrates. We have used this information to design herbicidal alpha- and gamma-substituted analogs of phosphinothricin (l-2-amino-4-(hydroxymethylphosphinyl)butanoic acid, PPT), a naturally occurring GS inhibitor and a potent herbicide. The substituted phosphinothricins inhibit cytosolic sorghum GS(1) and chloroplastic GS...

OBJECTIVE An adverse intrauterine environment indicated by both low birth weight and monozygosity is associated with an age- or time-dependent reduction in glucose disposal and nonoxidative glucose metabolism in twins, suggesting impaired regulation of muscle glycogen synthesis. RESEARCH DESIGN AND METHODS We measured the activities of glycogen synthase (GS), GS kinase (GSK)3 alpha, GS phosph...

Experiments have revealed that in the β(2) adrenergic receptor (β(2)AR)-Gs protein complex the α subunit (Gαs) of the Gs protein can adopt either an "open" conformation or a "closed" conformation. In the "open" conformation the Gs protein prefers to bind to the β(2)AR, while in the "closed" conformation an uncoupling of the Gs protein from the β(2)AR occurs. However, the mechanism that leads to...

Glutaredoxin (GRx, thioltransferase) is implicated in cellular redox regulation, and it is known for specific and efficient catalysis of reduction of protein-S-S-glutathione-mixed disulfides (protein-SSG) because of its remarkably low thiol pK(a) ( approximately 3.5) and its ability to stabilize a catalytic S-glutathionyl intermediate (GRx-SSG). These unique properties suggested that GRx might ...