4F is an anti-inflammatory, apolipoprotein A-I (apoA-I)-mimetic peptide that is active in vivo at nanomolar concentrations in the presence of a large molar excess of apoA-I. Physiologic concentrations ( approximately 35 microM) of human apoA-I did not inhibit the production of LDL-induced monocyte chemotactic activity by human aortic endothelial cell cultures, but adding nanomolar concentration...

The rapidly growing world population has a greatly increasing demand for plant biomass, thus creating a great interest in the development of methods to enhance the growth and biomass accumulation of crop species. In this study, we used zinc finger artificial transcription factor (ZF-ATF)-mediated genome interrogation to manipulate the growth characteristics and biomass of Arabidopsis plants. We...

HIV-1 infects immature dendritic cells (iDCs), but infection is inefficient compared with activated CD4+ T cells and only involves a small subset of iDCs. We analyzed whether this could be attributed to specific cellular restrictions during the viral life cycle. To study env-independent restriction to HIV-1 infection, we used a single-round infection assay with HIV-1 pseudotyped with vesicular ...

In this study, we show that at least one lethal mutation at the 3F-4A region of the X chromosome can generate an array of chromosome rearrangements, all with one chromosome break in the 3F-4A region. The mutation at 3F-4A (secondary mutation) was detected in an X chromosome carrying a reverse mutation of an unstable lethal mutation, which was mapped in the 6F1-2 doublet (primary mutation). The ...

Our investigations started with the question, why are there so many VEGF isoforms? One possibility that was considered was that some isoforms may interact with receptors other than VEGFR1 and VEGFR2, the only VEGF receptors known at that time. Cross-linking studies using labeled VEGF165 and human umbilical vein endothelial cells (HUVEC) revealed two crosslinking products that were missing in cr...

INTRODUCTION Factor concentrates are currently available and becoming increasingly used off-label for treatment of bleeding. We compared recombinant activated factor VII (rFVIIa) with three-factor prothrombin complex concentrate (3F-PCC) for the ability to augment thrombin generation (TG) in neonatal plasma after cardiopulmonary bypass (CPB). First, we used a computer-simulated coagulation mode...

Some theoretical aspects of 3-flavour (3f) neutrino oscillations are reviewed. These include: general properties of 3f oscillation probabilities; matter effects in νμ ↔ ντ oscillations; 3f effects in oscillations of solar, atmospheric, reactor and supernova neutrinos and in accelerator long-baseline experiments; CP and T violation in neutrino oscillations in vacuum and in matter, and the proble...

The Hyers-Ulam stability, the Hyers-Ulam-Rassias stability, and also the stability in the spirit of Gǎvru̧ta for each of the following quadratic functional equations f(x+y)+ f(x−y) = 2f(x)+ 2f(y), f(x+y + z)+ f(x−y)+ f(y − z)+ f(z−x) = 3f(x)+3f(y)+3f(z), f (x+y+z)+f(x)+f(y)+f(z)= f(x+y)+f(y+z)+f(z+x) are investigated. 2000 Mathematics Subject Classification. Primary 39B52, 39B72, 39B82.

In the Chinese hamster lung cell line DC-3F/9-OH-E, selected for resistance to 9-OH-ellipticine and cross-resistant to other topoisomerase II inhibitors, the amount of topoisomerase IIalpha is 4-5-fold lower than in the parental DC-3F cells, whereas topoisomerase IIbeta is undetectable. Cloning and sequencing of topoisomerase IIalpha cDNAs from DC-3F and DC-3F/9-OH-E cells revealed an allele po...

Peripheral nerve growth is regulated by the coordinated action of numerous external stimuli, including positively acting neurotrophin-derived growth cues and restrictive semaphorin cues. Here, we show that Semaphorin 3F (Sema 3F) can antagonize nerve growth factor (NGF)-stimulated TrkA (tyrosine receptor kinase A) signaling in sympathetic neurons, thereby apparently contributing to growth cone ...