Mutant alleles of two genetic regulatory elements, which underlie a three- to sixfold reduction in beta-glucuronidase (GUS) activity levels, distinguish mice of the H haplotype from those of the other two common GUS haplotypes, A and B. Both elements are tightly linked to the GUS structural gene over which they exert control. One (Gus-u) exerts a cis-active effect upon GUS activity levels in al...

A β-glucuronidase variant, GUS-TR3337, that was obtained by directed evolution exhibited higher thermostability than the wild-type enzyme, GUS-WT. In this study, the utility of GUS-TR337 as an improved reporter was evaluated. The corresponding gus-tr3337 and gus-wt genes were independently cloned in a plant expression vector and introduced into Arabidopsis thaliana. With 4-MUG as a substrate, p...

VASA (VAS), a key protein in establishing the specialized translational activity of the Drosophila pole plasm, accumulates at the posterior pole of the developing oocyte. We identified a gene, gustavus (gus), that encodes a protein that interacts with VAS. A gus mutation blocks posterior localization of VAS, as does deletion of a segment of VAS containing the GUS binding site. Like VAS, GUS is ...

by CONRAD V. IBAÑEZ (Under the Direction of Eileen T. Kraemer) ABSTRACT The Genomics Unified Schema (GUS) is a relational database schema that supports a wide range of data types including genomics, gene expression, transcript assemblies, proteomics, and many others. Together with the GUS Application Framework and the GUS Web Development Kit (WDK), GUS is used by many genomics projects and grou...

Chimeric mice were produced by aggregating 2 embryos, each of which was homozygous for a different structural allele of the enzyme /?-glucuronidase. The two alleles used were Gus, the 'wild-type' allele, and Gus, an allele whose gene product shows decreased activity in all tissues as well as decreased heat stability. Staining of untreated adult chimeric livers for glucuronidase activity reveale...