This study was directed at the evaluation of the prevalence of bovine viral diarrhea virus (BVDV) infection in bulls in artificial insemination centers. Both serological and virological examinations were performed. Blood samples were tested in micro-seroneutralization test for BVDV antibodies. Virus isolation was performed in cell culture and BVDV antigen was detected in an indirect immunofluor...

This report is an overview of enterovirus epidemiology in Tunisia during a 12-year period from 1992 to 2003. A total of 4700 clinical samples were collected as part of the national poliovirus surveillance programme and the routine diagnostic programme for aseptic meningitis. Enterovirus detection was performed by isolation on cell culture according to World Health Organization recommended proto...

A therapeutic anti-rabies immunoglobulin for human use has been produced mainly in horses. The presently available seroneutralization test, the rapid fluorescent focus inhibition test (RFFIT), is laborious and rather difficult to carry out in horse farms. This study was undertaken to develop a simple latex agglutination test (LAT) for determining rabies antibodies in horse sera. LAT was validat...

Thirty-three pestiviruses isolated from bovines on different farms in Chile were characterized at the molecular level. The 5'-untranslated region (5'UTR) of the isolates was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and subsequently digested with restriction enzymes (RE) Bgl I, Xho I and Pst I. Furthermore, the isolates were amplified by differential RT-PCR, which se...

Sugiyama live attenuated measles vaccine produced in bovine renal ceIls was administered during pilot studies and a mass immunization programme in Iran. Clinical observations in several of the vaccinees revealed a mild fever lasting 3-4 days which was accompanicd by the appearance of a morbilIiform rash which faded in 2 days. There was no significant difference in rcactions from subcutaneous or...

A Zika virus seroepidemiology study was performed in 1084 blood donors collected from August to October 2015 in six sites of Cameroon representing a large panel of eco-environments. Samples were tested using an anti-NS1 IgG ELISA detection kit and positives were further confirmed by seroneutralization. The observed global seroprevalence was low (around 5%, peaking at 10% and 7.7% in Douala and ...

Botulism is a disease usually fatal, caused by the ingestion of neurotoxins produced Clostridium botulinum. In dogs, intoxication botulinum toxin type C, and animals often recover spontaneously. The present study describes occurrence C botulism in two dogs domiciled on neighboring rural properties municipality Goiânia, state Goiás, Brazil, probably associated with decomposing bovine carcass. Up...