Fem proteins are the essential structural proteins of various gram-positive bacteria. These are of three different types namely FemX (FmhB), FemA and FemB. Only two Fem protein crystallographic structures are available till date, one for FemA in Staphylococcus aureus and another for FemX in Weissella viridescensis. In this study, computational methods are used to evaluate interaction of FemA pr...

Protein-protein interactions play an important role in all cellular processes. The development of two-hybrid systems in yeast and bacteria allows for in vivo assessment of such interactions. Using a recently developed bacterial two-hybrid system, the interactions of the Staphylococcus aureus proteins FemA, FemB and FmhB, members of the FemABX protein family, which is involved in peptidoglycan b...

This paper comprehensively evaluates the Modal Pushover Analysis (MPA) procedure against the ‘‘exact’’ nonlinear response history analysis (RHA) and investigates the accuracy of seismic demands determined by pushover analysis using FEMA-356 force distributions; the MPA procedure in this paper contains several improvements over the original version presented in Chopra and Goel (2002). Seismic de...

This is one of a series of audit, inspection, and special reports prepared as part of our oversight responsibilities to promote economy, efficiency, and effectiveness within the department. This report addresses FEMA's preparedness for the next catastrophic disaster. It is based on interviews with employees and officials of relevant agencies and institutions, direct observations, and a review o...

The growth of A-1 fibroblasts depends on exogenous amyloid beta/A4 protein precursor (APP), providing a simple bioassay to study the function of APP. Our preliminary study, testing the activity of a series of fragments derived from the secreted form of APP-695 (sAPP-695) on this bioassay, has shown that at least one of the active sites of sAPP-695 was localized within a 40-mer sequence (APP296-...