نتایج جستجو برای: تیوردوکسین nadp
تعداد نتایج: 13985 فیلتر نتایج به سال:
Several microorganisms can utilize l-rhamnose as a carbon and energy source through the nonphosphorylative metabolic pathway, in which 1-dehydrogenase (RhaDH) catalyzes NAD(P)+-dependent oxidization of to l-rhamnono-1,4-lactone. We herein investigated crystal structures RhaDH from Azotobacter vinelandii ligand-free, NAD+-bound, NADP+-bound, l-rhamnose- NAD+-bound forms at 1.9, 2.1, 2.4, 1.6 Å r...
interaction of glucose 6-phosphate dehydrogenase from s. aureofaciens with nad+, nadp+and glucose 6-phosphate were investigated using different fluorescent probes. binding of nad+, nadp+and s-nadph to the native enzyme quenched intrinsic protein fluorescence by 100%, 10% and 21%,respectively, from which kd values of nad+ (6.5 mm), nadp+ (92.0 μm) and s-nadph (122.0 μm)were calculated. binding o...
The Arabidopsis (Arabidopsis thaliana) genome contains four genes encoding putative NADP-malic enzymes (MEs; AtNADP-ME1-ME4). NADP-ME4 is localized to plastids, whereas the other three isoforms do not possess any predicted organellar targeting sequence and are therefore expected to be cytosolic. The plant NADP-MEs can be classified into four groups: groups I and II comprising cytosolic and plas...
Purified potato tuber (Solanum tuberosum L. cv Bintie) mitochondria contain soluble, highly latent NAD(+)- and NADP(+)-isocitrate dehydrogenases, NAD(+)- and NADP(+)-malate dehydrogenases, as well as an NADPH-specific glutathione reductase (160, 25, 7200, 160, and 16 nanomoles NAD(P)H per minute and milligram protein, respectively). The two isocitrate dehydrogenase activities, but not the two m...
تیوردوکسین ها پروتئین هایی فراگیر و از لحاظ وزن مولکولی کوچک (معمولا شامل 100 تا 120 آمینو اسید) بوده و دارای پل دی سولفیدی انفعالی با توالی های کاملا حفاظت شده - –cys- gly (ala/pro)- pro- cys می باشند. در این تحقیق، پس از استخراج rna کل ازبافت های برگ و حبه انگور (vitis vinifera l.) رقم یاقوتی، سنتز cdna ازrna استخراجی صورت پذیرفت. ژن تیوردوکسین h تحت عنوان vvtrxh ، با استفاده از تکنیک واکنش ز...
تیوردوکسین ردوکتاز آنزیمی با عملکرد های متعدد از قبیل شرکت در واکنش های احیا کننده، سنتز پروتئین، سنتز dna، تنظیم عملکرد فاکتورهای رونوشت برداری، تنظیم آپوپتوزیس، و تنظیم فعالیت سیستم ایمنی می باشد . تیوردوکسین ردوکتاز در گونه های متعددی تعیین توالی شده است. توالی ژن تیوردوکسین ردوکتاز در گوسفند مشخص نیست و گزارشی در این مورد وجود ندارد. نقش این آنزیم نیز در گوسفند نامشخص است. در این بررسی تلاش...
In plants, the NADP malic enzymes (NADP-MEs) are encoded by small gene families. These NADP-ME gene families are relatively well described in C4 plants but not well studied in C3 plants. In this study, we investigated the NADP-ME gene family in a model C3 monocot plant (rice, Oryza sativa) based on its recently released genomic DNA sequence. We found that the rice NADP-ME family is composed of ...
Modification of metabolic pattern by variation of nicotinamide adenine dinucleotide phosphate level.
The experiments were designed to get some information on the metabolism controlled by variation of the NADP level, which is known to change with the variation of environmental factors.The exogenous NADP added to the mitochondria prepared from Vigna sesquipedalis cotyledons was associated with and/or penetrated into the mitochondria. The combined NADP served in the operation of the mitochondrial...
A pyridine nucleotide has been synthesized from cr-nicotinamide adenine dinucleotide and adenosine triphosphate with the use of a crude enzyme preparation from Azotobacfer uinelandii strain 0. Evidence is presented that this nucleotide is the (Y isomer of NADP, ar-NADP, which differs from &NADP in the configuration of the nicotinamide-ribosidic linkage. This evidence rests on a study of (a) spe...
A pyridine nucleotide has been synthesized from cr-nicotinamide adenine dinucleotide and adenosine triphosphate with the use of a crude enzyme preparation from Azotobacfer uinelandii strain 0. Evidence is presented that this nucleotide is the (Y isomer of NADP, ar-NADP, which differs from &NADP in the configuration of the nicotinamide-ribosidic linkage. This evidence rests on a study of (a) spe...
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