Recent studies have shown that point mutations in the dihydropteroate synthase (DHPS) gene of human-derived Pneumocystis carinii are related to exposure to sulfa drugs and possibly represent the emergence of sulfa resistance. We developed a simple single-strand conformation polymorphism (SSCP) method to permit rapid detection of these mutations. With plasmid constructs, SSCP was able to detect ...

Background: Cellular tolerance to heat stress is mediated by shock proteins (HSPs). The HSPs act as molecular chaperones and are transcribed in response stress. Among different families of these proteins, HSP70 considered be related the development temperature tolerance. Unraveling polymorphism protein genes could a step towards identification genetic markers for selecting heat-tolerant cattle....

Toxin-producing Escherichia coli expressing F18 fimbriae colonizes the small intestines of weaned pigs and causes diarrhea, edema disease, or both. The F18 family is composed of two antigenic variants, F18ab and F18ac. Because many strains do not express F18 fimbriae in vitro, identification and differentiation of these two variants are difficult. Single-strand conformational polymorphism (SSCP...

The aim of the study was to develop a reliable and cost-effective method for detection nonsense mutation in APAF1 gene causing lethal effect called HH1 (Holstein Haplotype1) evaluate its prevalence sample Polish Holstein-Friesian bulls. One hundred seventy eight bulls born between 1996 2017 were included analysis. They kept four artificial insemination centers have pedigree known carrier HH1. A...

Abstract Background Stigma associated with mental disorders is rooted among many pharmacists, and represents a major barrier to patient support in community-based psychiatry. We developed an assessment scale that specifically designed assess the level of stigma pharmacists may have toward schizophrenia, then examined effects reducing educational program focuses on communication patients diagnos...

This study was conducted to assess the possible association between polymorphisms of transcription factor 7-like 2 (TCF7L2) gene and obese Iraqi adults. DNA obtained from 158 subjects 142 controls. Two specific PCR fragments were designed incorporate two highly frequent single nucleotide polymorphism (SNP)s within TCF7L2, rs11196208 rs7908486. Both amplified loci genotyped by PCR-single-strand ...

We report optimization of single-strand conformation polymorphism (SSCP) analysis in the presence of polyethylene glycol. The protocol developed separates single-strand conformers in a much shorter time (1-3 h) than conventional SSCP protocols and broadens the applicability of SSCP analysis from 150 to as much as 500 bp of DNA by different percentages of GC content present. We conclude that add...

DOI: 10.1002/sscp.202100018 The cover picture shows a densitogram of base hydrolyzed sample Ibrutinib that has well separated drug and degradant peak.

AIMS The primary objectives of this study were to determine if a single-strand conformation polymorphism (SSCP) analysis can be used for rapid identification of Phytophthora ramorum, an important quarantine plant pathogen worldwide, and to further assess the potential of the SSCP technique as a taxonomic tool for the genus Phytophthora. METHODS AND RESULTS SSCP of ribosomal DNA internal trans...

Capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) is a promising high-throughput tool for profiling complex bioremediation communities, but has not been well characterized with respect to other methods such as denaturing gradient gel electrophoresis (DGGE). The purpose of this study was to compare CE-SSCP with DGGE with respect to: 1) overall representation of the comm...