Incorporation of nonnatural amino acids into proteins is a powerful technique in protein research. Amber suppression has been used to this end, but this strategy does not allow multiple incorporation of nonnatural amino acids into single proteins. In this article, we developed an alternative strategy for nonnatural mutagenesis by using four-base codons. The four-base codons AGGU, CGGU, CCCU, CU...

T he p reny la ted isoflavone, 2 ,3-dehydrokievitone [5 ,7 ,2 ',4 '-tetrahydroxy-8-(3 ,3-dim ethylally l)isoflavone, 1], was m etabolized by both Aspergillus flavus and Botrytis cinerea to yield the sam e th ree com pounds, a dihydrofurano-isoflavone (D K -M 1, 2), a d ihydropyrano-isoflavone (D K M 2, 3), and a glycol (D K -M 3, 4). A lthough structure 3 has previously been assigned to lunaton...

The yield of J/ψ per binary nucleon-nucleon collision in AuAu and CuCu collisions at √ s = 200 GeV is computed in the framework of the dual parton model, supplemented with final state interaction (comovers interaction). For the latter we use the same value of the cross-section, σco = 0.65 mb, which describes the anomalous J/ψ suppression observed at CERN-SPS energies. Several possibilities for ...