The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carried out in mammalian COS-7, CHO-K1 and HeLa cells to facilitate investigation ...

PURPOSE Activation of the MDR1 upstream promoter (USP) has been described previously in four lymphoblastic leukemia patients, where it is the major MDR1 promoter associated with P-glycoprotein overexpression. We asked whether MDR1 USP-derived transcripts were also present in breast carcinoma and assessed their potential as a biomarker. EXPERIMENTAL DESIGN We developed a sensitive method for d...

Ultra-short-period (USP) planets are a newly recognized class of planets with periods shorter than one day, and radii smaller than about 2 R⊕. It has been proposed that USP planets are the solid cores of hot Jupiters that lost their gaseous envelopes due to photo-evaporation or Roche lobe overflow. We test this hypothesis by asking whether USP planets are associated with metal-rich stars, as ha...

1 PhD, Medical Sciences (MD). 2 PhD, Otorhinolaryngology (Assistant Physician, HCRP-FMRP USP). 3 Professor (Retired Professor, FMRP USP). 4 PhD, Medicine (Professor, FMRP-USP). Send correspondence to: Miguel Angelo Hyppolito. Av. Bandeirantes, no 3900, 12o andar. Monte Alegre. Ribeirão Preto SP. Brazil. CEP: 14048-900. Medical School of Ribeirão Preto University of São Paulo University Hospital...

Pulses of the steroid hormone ecdysone function as key temporal signals during insect development, coordinating the major postembryonic developmental transitions, including molting and metamorphosis. In vitro studies have demonstrated that the EcR ecdysone receptor requires an RXR heterodimer partner for its activity, encoded by the ultraspiracle (usp) locus. We show here that usp exerts no app...

BACKGROUND Bacterial genotoxins provoke DNA damage and carcinogenesis. The Escherichia coli uropathogenic-specific protein gene, usp, and its linked genes, imu1-3, are associated with strains from pyelonephritis, prostatitis, and bacteremia of urinary tract origin. While the Usp C-terminal domain exhibits similarity with DNase-like colicins and pyocins, its role and mechanisms of action, as wel...

The nuclear receptor complex for the insect steroid hormone, 20-hydroxyecdysone (20E), is a heterodimer of EcR and USP. It has been shown that Drosophila EcR and USP can be sumoylated in mammalian cells, but it is unknown whether EcR-USP sumoylation naturally occurs in Drosophila. In Drosophila cells, USP, but not EcR, was sumoylated by Smt3, the only Drosophila SUMO protein. The presence of Ec...

1 Enfermeira do Hospital das Clínicas Faculdade de Medicina de Ribeirão Preto-USP. Professora do Centro Universitário Barão de Mauá. Mestranda da Escola de Enfermagem de Ribeirão Preto-USP. 2 Enfermeira Professora Doutora do Departamento de Enfermagem Psiquiátrica e Ciências Humanas da Escola de Enfermagem de Ribeirão Preto-USP. 3 Enfermeira. Professora do Departamento de Enfermagem Psiquiátric...

1) DDS, Universidade de São Paulo (USP), School of Dentistry (FOUSP), São Paulo, São Paulo, Brazil. 2) Professor of Removable Partial Prosthesis, Universidade de São Paulo (USP), School of Dentistry (FOUSP), São Paulo, São Paulo, Brazil. 3) Phd resident in Dental Prosthesis, Universidade de São Paulo (USP), School of Dentistry (FOUSP), São Paulo, São Paulo, Brazil. 4) Full professor, Universida...

Study carried out at Centro Saúde Escola, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo – USP, Ribeirão Preto (SP), Brazil. 1 Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo – USP, Ribeirão Preto (SP), Brazil. 2 Undergraduate, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo – USP, Ribeirão Preto (SP), Brazil....