A pyridine nucleotide has been synthesized from cr-nicotinamide adenine dinucleotide and adenosine triphosphate with the use of a crude enzyme preparation from Azotobacfer uinelandii strain 0. Evidence is presented that this nucleotide is the (Y isomer of NADP, ar-NADP, which differs from &NADP in the configuration of the nicotinamide-ribosidic linkage. This evidence rests on a study of (a) spe...

A pyridine nucleotide has been synthesized from cr-nicotinamide adenine dinucleotide and adenosine triphosphate with the use of a crude enzyme preparation from Azotobacfer uinelandii strain 0. Evidence is presented that this nucleotide is the (Y isomer of NADP, ar-NADP, which differs from &NADP in the configuration of the nicotinamide-ribosidic linkage. This evidence rests on a study of (a) spe...

NADPH is a pivotal molecule in oxidative stress, during which it is potentially produced by several cytosolic NADP-linked dehydrogenases. This study investigated the response and functional importance of the major leaf cytosolic NADP-malic enzyme in Arabidopsis (NADP-ME2) during oxidative stress. Data from both microarray and targeted quantitative PCR analyses showed that NADP-ME2 transcripts a...

Human glucose-6-phosphate dehydrogenase (G6PD) is NADP(+)-dependent and catalyses the first and rate-limiting step of the pentose phosphate shunt. Binary complexes of the human deletion mutant, DeltaG6PD, with glucose-6-phosphate and NADP(+) have been crystallized and their structures solved to 2.9 and 2.5 A, respectively. The structures are compared with the previously determined structure of ...

‘Xiangjiao’ plum (Prunus salicina Lindl.) is a stone fruit that vulnerable to the chilling injury (CI) caused by low-temperature stress. The effects of 1-methylcyclopropene (1-MCP) and ethylene absorbent (EA) treatments on quality malic acid metabolism stored at 4 °C were compared in this study. Compared with control check (CK) EA treatment, fumigation 1.0 mg·L−1 1-MCP for 24 h could more signi...

Eukaryotic metabolism is organised in complex networks of enzyme catalysed reactions which are distributed over different organelles. To quantify the compartmentalised reactions, quantitative measurements of relevant physiological variables in different compartments are needed, especially of cofactors. NADP(H) are critical components in cellular redox metabolism. Currently, available metabolite...

هدف: هدف این پژوهش مقایسه اثربخشی امیددرمانی و معنویت درمانی بر کیفیت زندگی، قند خون فشار زنان میانسال مبتلا به دیابت نوع دو بود. روش: حاضر نیمه آزمایشی با طرح پیش آزمون- پس پیگیری گروه گواه جامعه آماری شامل تمام 2 مراجعه کننده مرکز تحقیقات دیابت، متابولیسم غدد شهر تهران در سال 1399 بودند که ‏45 نفر شیوه نمونه‌گیری دسترس انتخاب صورت تصادفی آزمایش یک (هر 15 نفر) قرار گرفتند. ابزار پرسشنامه کوتاه...

Glucose 6-phosphate dehydrogenase (G6PD) was purified from Streptomyces aureofaciens and inactivated with butanedione and diethylpyrocarbonate. Incubation of the enzyme with butanedione resulted in a rapid activity loss (80%) within 5 min, followed by a slow phase using a molar ratio to enzyme concentration of 100. Fluorescence studies showed a conformational change in the butanedione-modified ...

NADP(+)-isocitrate dehydrogenase (NADP(+)-IDH) from the dinitrogen-fixing filamentous cyanobacterium Anabaena sp. strain PCC 7120 was purified to homogeneity. The native enzyme is composed of two identical subunits (M(r), 57,000) and cross-reacts with antibodies obtained against the previously purified NADP(+)-IDH from the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. Anabaena N...

NADP-dependent isocitrate dehydrogenase (NADP-ICDH) is an important enzyme involved in energy metabolism. The complete coding sequence of the pepper (Capsicum annuum) NADP-ICDH gene was amplified using a reverse transcriptase PCR based on the conserved sequence information of the tomato and other Solanaceae plants and known highly homologous pepper ESTs. Nucleotide sequence analysis reveal...