The blood serum of the European flounder Platichthys flesus strongly inhibits soluble erythrocytic carbonic anhydrase from the same species. The inhibition is of the uncompetitive type. Hence, the mechanism of the carbonic anhydrase inhibition is different from that of all other known carbonic anhydrase inhibitors. The serum showed no inhibitory effect on carbonic anhydrase from human and bovin...

Carbonic anhydrase isozymes, carbonic anhydrase I and II, also known as carbonate dehydratase, were isolated and purified from human erythrocytes. Rabbits were then immunized and the respective types of antisera were prepared. Using the antisera obtained, the carbonic anhydrase I and II levels in erythrocytes of healthy persons and patients with chronic glomerulonephritis, primary nephrotic syn...

OBJECTIVES To determine the serum levels of methylglyoxal (MG) and carbonic anhydrase (CA) activity in known cases of type-II diabetes mellitus with insulin resistance and to compare them with age-sex matched healthy controls. METHODS Eightysix participants were enrolled for the present case-controlled study with ages ranging from 25 to 44 years. The analytes measured were fasting blood gluco...

the ph dependence study reveals that the cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. during the reaction of 2,2'-dithiobispyridine (2-dtp) with the enzyme, there was no absorbance change recorded. in the presence ofsurfactants, the ph dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-dtp with bovine carbonic a...

the ph dependence study reveals that the cys 206 sulphydryl group of bovine carbonicanhydrase in the native form is not exposed. during the reaction of 2,2'-dithiobispyridine (2-dtp) with the enzyme, there was no absorbance change recorded. in the presence ofsurfactants, the ph dependence profiles of the apparent second order rate constants, kapp, forthe reaction of 2-dtp with bovine carbo...

Carbamoyl phosphate has been shown to inhibit carbonic anhydrase (CA) isozymes CA I, CA II and CA III. This physiologically important molecule is the most potent, naturally occurring inhibitor of carbonic anhydrase yet found. It is also unique, among carbonic anhydrase inhibitors discovered hitherto, in that it inhibits the 3 isozymes with equal effect, despite their strikingly different proper...

Carbonic anhydrase activity was characterized in freshly dissociated Müller cells of the salamander retina. Intracellular pH was monitored using ratio imaging of the indicator dye BCECF as extracellular PCO2 was varied. The extracellular solution was switched rapidly (141 ms rise time) from a HEPES buffered to a CO2-HCO3- buffered solution (both pH 7.4). Introduction of CO2-HCO3- produced a rap...

A physiologically significant level of intracellular carbonic anhydrase has been identified in Chlamydomonas reinhardtii after lysis of the cell wall-less mutant, cw15, and two intracellular polypeptides have been identified which bind to anti-carbonic anhydrase antisera. The susceptibility of the intracellular activity to sulfonamide carbonic anhydrase inhibitors is more than three orders-of-m...

Keywords: 5-amino-1,3,4-thiadiazole-2-thiol derivatives, diuretic activity, saluretic/natriuretic/carbonic anhydrase inhibition indexes, SAR,

We investigated the activity of carbonic anhydrase in blood-free perfused white skeletal muscles of the rabbit. Carbonic anhydrase activities were measured in supernatants and in Triton extracts of the particulate fractions of white-skeletal-muscle homogenate by using a rapid-reaction stopped-flow apparatus equipped with a pH electrode. An average carbonic anhydrase concentration of about 0.5 m...