In a survey on the biodiversity of Penicillium species in soils of the National Park of Urmia Lake (NW Iran), eight Penicillium isolates were isolated as members of the section Citrina. Based on a combination of cultural and morphological criteria, the isolates were identified as Penicillium anatolicum, P. sanguifluum,and P. sizovae. The identity of each species was further confirmed using sequ...

Objective(s): In this study a series of novel colchicine-like β-acetamidoketones was designed and synthesized as potential tubulin inhibitorsMaterials and Methods: The cytotoxicity of the novel synthesized β-acetamidoketones was assessed against two cancerous cell lines including MCF-7 (human breast cancer cells) and A549 (adenocarcinomi...

PURPOSE Peripheral neuropathy is the dose-limiting toxicity of paclitaxel, a chemotherapeutic drug widely used to treat several solid tumors such as breast, lung, and ovary. The cytotoxic effect of paclitaxel is mediated through β-tubulin binding in the cellular microtubules. In this study, we investigated the association between paclitaxel neurotoxicity risk and regulatory genetic variants in ...

More than 20 years ago, biochemical analysis of the eukaryotic cell cytoskeleton revealed the major component proteins. The heterodimeric (α/β) protein tubulin was defined as the building block of microtubules, assembled in a polar manner into specifically arranged protofilaments in the microtubule wall [1]. The next two members of the tubulin protein superfamily were both discovered by genetic...

The aim of this study was to explore the correlation between mRNA expression of β-tubulin-III and stathmin in patients with stage II non-small cell lung cancer (NSCLC) and the chemosensitivity to Navelbine plus cisplatin (NP), as well as to provide a basis for personalized treatment. A single-gene quantitative test was performed to detect the mRNA expression of β-tubulin-III and stathmin in the...

The real-time quantitative reverse transcription PCR (RT-qPCR) is widely used for gene expression analysis, owing to its advantages of high specificity, sensitivity and repeatability. Suitable reference an absolute prerequisite accurate normalisation. In this study, three computational statistical methods before performing RT-qPCR, including geNorm, NormFinder BestKeeper was integrated stabilit...

We report the powdery mildew Golovinomyces ambrosiae for first time on silverleaf nightshade (Solanum azureum), a host plant endemic to northern Sinaloa and southern Sonora, Mexico. No teleomorph was observed, requiring fungal species be identified based morphological characteristics of anamorph. Phylogenetic analyses ITS region partial sequence β-tubulin gene from four specimens confirmed iden...

The study investigated the clinical significance of RRM1 (ribonucleoside reductase subunit M1), TUBB3 (tubulin-β-III), TOP2A (DNA topoisomerase II), CYP19A1 (cytochrome P450, family 19, subfamily A, polypeptide 1) and CYP2D6 (cytochrome P450, family 2, subfamily D, polypeptide 6) for the diagnosis and possible predictive roles in breast cancer. Tissue microarray detected the expression of RRM1,...

microtubules are unique cytoskeletal structures that have structural subunits of αβ tubulin. taxol is a typical microtubule stabilizing drug. the epothilones are other natural products with similar mechanism of action totaxol. despite the highly conserved nature of β-tubulin, some organism like saccharomyces cerevesia is resistance to taxol, but sensitive to epothilones. in order to find differ...

Control of helminth infections is a major task in livestock production to prevent health constraints and economic losses. However, resistance to established anthelmintic substances already impedes effective anthelmintic treatment in many regions worldwide. Thus, there is an obvious need for sensitive and reliable methods to assess the resistance status of at least the most important nematode po...