OBJECTIVES To compare the ability of different cyclodextrin polysulphate (CDPS) derivatives to affect human articular cartilage cell metabolism in vitro. METHODS OA chondrocytes were cultured in alginate and exposed to 5 µg/ml of 2,3,6-tri-O-methyl-β-cyclodextrin (ME-CD), 2,3-di-O-methyl-6-sulphate-β-cyclodextrin (ME-CD-6-S), 2,6-di-O-methyl-3-sulphate-β-cyclodextrin (ME-CD-3-S), (2-carboxyet...

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

in this paper, synthesized (raw) multi-walled carbon nanotube (rmwcnts) surfaces were modified with chitosan and β-cyclodextrin using chen’s soft cutting technique. raw and surface modified multi-walled carbon nanotubes were observed by transmission electron microscope (tem). the results showed that chitosan and β-cyclodextrin could attach to the outer surface of nanotubes, wrapping the nanotub...

Ephedrine and pseudoephedrine can be used as precursors for the illicit synthesis of stimulant drugs, such methamphetamine methcathinone. For this reason, an enantioselective capillary electrokinetic chromatography method was developed separation ephedrine enantiomers. After testing two different charged or chargeable cyclodextrins (β-cyclodextrin sulphate carboxymethyl-β-cyclodextrin) a few mi...

The cyclodextrin glucosyltransferase enzyme (CGTase) is an industrially crucial for the production of β-cyclodextrin (β-CD). CGTase has a high propensity to produce mixture cyclodextrins (CDs). From industrial perspective, that produces only one type CD critical importance. Bacillus sp. PBS1 produced converts starch solely into β-CD. isolated strain was found close similarity with alkaliphilic ...

Sonochemical procedure was used to synthesize Co/β-cyclodextrin complex. For the first time, Fe/β-cyclodextrin complex also synthesized in alkaline media. Moreover, β-cyclodextrin modified by citric acid (CA) and reacted with metal salts under ultrasonic irradiation. The prepared catalysts were characterized using EDX, UV–vis FTIR analytical techniques. highest incorporation of into observed cr...

The production of β-maltooligosaccharides of capsaicin was investigated using Lactobacillus delbrueckii and cyclodextrin glucanotransferase (CGTase) as biocatalysts. The cells of L. delbrueckii glucosylated capsaicin to give its β-glucoside. The β-glucoside of capsaicin was converted into the corresponding β-maltoside and β-maltotrioside by CGTase. On the other hand, β-melibioside and β-isomalt...

A simple and cost-effective method for the synthesis of sulfated β-cyclodextrin, one most widely used chiral mobile phase additives, using sulfamic acid as a sulfonating agent has been described. The was optimized, synthesized product characterized by spectroscopic, size-exclusion chromatographic, thermal, microscopic methods compared to marketed Sigma Aldrich β-cyclodextrin. β-Cyclodextrin, hy...

Background: The complexity of the fermentation processes is mainly due to the complex nature of the biological systems which follow the life in a non-linear manner. Joined performance of artificial neural network (ANN) and genetic algorithm (GA) in finding optimal solutions in experimentation has found to be superior compared to the statistical methods. Range of applications of β-cyclodextrin (...

Selective visualization of arginine and lysine has been explored among 20 amino acids using the hybrid conjugate of β-cyclodextrin (β-CD) and polydiacetylene (PDA). The mono pentacosa-10,12-diynyl aminomethyl group was successfully coupled to either the primary or the secondary face of β-CD, where mono-6-amino-6-deoxy-β-CD or mono-3-amino-3-deoxy-β-CD reacted with the N-hydroxysuccinimide ester...