Gene transfer with vectors derived from murine retroviruses is re stricted to cells which are proliferating and synthesizing DNA at the time of infection. This suggests that retroviral-mediated gene transfer might permit targeting of gene integration into malignant cells in organs com posed mainly of quiescent nonproliferating cells, such as in the brain. Accordingly, selective introduction of ...

Introduction: The management of children with type 1 diabetes has been revolutionized first by the discovery insulin and then development new technologies. Nevertheless, majority patients still do not reach their glycemic target. main objective this study is to determine predictive factors good metabolic control in I diabetes, order develop tailored approaches improve quality prevent disease-re...

Heparin inhibited the activities of: (a) reverse transcriptase from simian sarcoma virus; (b) DMA polymerases a, (i, and y from phytohemagglutinin-stimulated normal hu man lymphocytes, human leukemic lymphoblasts, and Molt-4 cells which are of acute lymphoblastic leukemia origin; and (c) terminal deoxynucleotidyl transferase from the two latter cell types. A 10-fold excess of enzyme overcame he...

Organic isothioc.vanates block the production of tumors induced in rodents by diverse carcinogens (polve-velie aromatic hydrocarbons, ¡i/o dyes, ethionine, jV-2-fluorenylacetamide, and nitrosamines). Protection is afforded by a-naphthyl-, ß-naphthyl-, phenyl-, benzyl-, phenethyl-, and other arylalkyl isothioc.vanates against tumor development in liver, lung, mammary gland, forestomach, and es...

l-j8-D-Arabinofuranosylcytosine (ara-C), an important antitumor agent, must be converted to the nucleotide by deoxycytidine kinase (K) in order to be biologically active. It is inactivated by ara-C deaminase (D) to \-ß-Darabinofuranosyluracil. The enzymes are widely distributed in normal and neoplastic tissues of both man and mouse. Low K and high D activities, thus low ratios of K to D, were ...

In an attempt to better understand breast tumors sensitivity or resis tance to anticancer drugs, the main drug-metabolizing enzyme systems were evaluated in both breast tumors and their corresponding peritumoral tissues in 12 patients. The following enzymes were assayed by Western blot: cytochromes P-4SO (1A1/A2, 2B1/B2, 2C8-10, 2E1, 3A4); glutathione .S'-transferases (GST-a, -u, and -TI); and ...

Acid hydrolase activities were measured in separated lymphocytes, polymorphonuclear leukocytes, and the total leukocyte population of normal and tumor-bearing rats. Only aryl sulfatase activity of the lymphocytes from tumor-bearing rats was significantly increased. Those showing significant decreases in tumor bearers were cathepsin in polymorpho nuclear leukocytes and ß-galactosidaseand acid p...

Ornithine decarboxylase (ODO plays a rate-limiting role in polyamine biosynthesis and is intimately associated with cell proliferation and func tion. Although elevated levels of ODC mRNA, protein, and enzyme activity are consistently detected in transformed cells and tumors, the question remains as to whether ODC gene overexpression has a causative role in tumorigenesis. We have stably transfec...

Nonneoplastic cell lines are unable to grow in soft agar. However, concomitant treatment of these cells with epidermal growth factor and transforming growth factor ßconfers upon them anchorage independence. Since articular chondrocytes are unique as normal diploid cells that do have the capability of growing in soft agar, we tested whether transforming growth factor ßand epidermal growth fact...

Cellular senescence is a programmed cell response leading to growth arrest in human diploid fibroblasts. We have shown that a nasopharyngeal carcinoma cell line, CNE1, following treatment by the DNA-damaging agent cisplatin, can undergo cellular senescent-like growth arrest, similar to fibroblasts, judged by cellular morphological changes and the expression of senescence-associated ß-galactosi...