The aim of this experiment was to evaluate the direct effect of genistein, daidzein and zearalenone on basal and hormone-induced lipogenesis and lipolysis in isolated rat adipocytes. In lipogenesis, daidzein and zearalenone were used at concentrations of 0.01, 0.1 and 1 mmol x L(-1) and genistein at concentrations of 0.01, 0.3, 0.6 and 1 mmol x L(-1). In lipolysis, concentrations of tested comp...

Zearalenone contamination of 175 wheat samples produced in 2004 in Golestan province, northern Iran was determined. The 10-20 kg samples collected at different stages, including pre-harvest, before and after storage for 2 months in local silos. Samples were ground and sub-sampled with an analytical Romer mill. Each sample extract was cleaned-up using an immunoaffinity column. Zearalenone levels...

Maize silage is a widely used feed product for cattle worldwide, which may be contaminated with mycotoxins, pre- and post-harvest. This concerns both farmers and consumers. To assess the exposure of Danish cattle to mycotoxins from maize silage, 99 samples of whole-crop maize (ensiled and un-ensiled) were analyzed for their contents of 27 mycotoxins and other secondary fungal metabolites by liq...

The levels of 26 mycotoxins were determined in 147 samples of the grain of cereals cultivated in five regions of Poland during the 2014 growing season. The HPLC-HRMS (time-of-flight) analytical technique was used. An analytical procedure to simultaneously determine 26 mycotoxins in grain was developed, tested and verified. Samples from eastern and southern Poland were more contaminated with myc...

The aim of the present study was to examine the abilities of twenty-four isolates belonging to ten different Trichoderma species (i.e., Trichoderma atroviride, Trichoderma citrinoviride, Trichoderma cremeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningiopsis, Trichoderma longibrachiatum, Trichoderma longipile, Trichoderma viride and Trichoderma viridescens) to inhibit the myce...

Glutathione S-transferase (GST) activity pattern was determined in tolerant (cv. Sumai#3) and susceptible (cv. Falat) wheat heads inoculated with Fusarium graminearum, the causal agent of head scab disease (FHB), during various developmental stages. GST specific activity exhibited a transient pattern in Sumai#3 reaching a maximum level at the milk stage and declining thereafter. GST level in Su...

mycotoxins are small, toxic chemical products produced as secondary metabolites by a few fungal spices that readily colonise crops and contaminate them with toxins in the field or after harvest. in this study a liquid chromatographic method was developed for the simultaneous determination of aflatoxins (afs) (b 1 , b 2 , g 1 , and g2), ochratoxin a (ota) and zearalenone(zea) toxins in wheat. fo...

New emerging strains of toxigenic molds in agricultural areas and insufficient data on levels their toxic metabolites occurring domestic horticultural fruits berries require risk assessment MT contamination for this plant group mass consumer products. This study concentrated samples fresh sold the market (185 samples, including 127 intact 58 with signs deformation molding). We applied our own d...