Oxygen uptake was measured in synchronous cultures of the fission yeast Schizosaccharomyces pombe. The rate of oxygen uptake was found to increase in a step-wise manner at the beginning of the cycle and again in the middle of the cycle. The increases in rate were such that overall, oxygen uptake doubled in rate once per cell cycle. Addition of inhibitors of DNA synthesis or nuclear division to ...

Strain PA-1 (S. Barik, W.J. Brulla, and M.P. Bryant, Appl. Environ. Microbiol. 50:304-310, 1985) is an anaerobic, gram-negative rod that in pure culture decarboxylates succinate to propionate and that grows syntrophically as an acetogen with the H2 utilizer Methanospirillum hungatei if glucose, pyruvate, aspartate, or fumarate is provided. In pure culture, strain PA-1 grows optimally in a mediu...

This study focused on the optimization of xylitol production from sugarcane bagasse by using response surface methodology. Xylitol was produced through a series processes, firstly, ultrasound-assisted mild alkaline pretreatment for xylan extraction followed enzymatic hydrolysis to xylose enzyme β-1,4-xylanase and finally microbial fermentation yeast (Candida guilliermondii), bacteria (Corynebac...

Pichia fermentans ITD-00165 is a yeast with high potential to produce isoamyl acetate, but no previous works report its growth in continuous culture. A bioreactor containing chemically defined medium allowed cultures develop three dilution rates (0.25, 0.30 y 0.35 h-1), measuring the acetate production. Steady-state concentration was measured using an entrapping system based on bubbling column ...

Three different strains of bacteria isolated from spoiled, uncooked chicken were grown in pure culture on Trypticase soy agar supplemented with yeast extract. The volatile organic compounds produced by each culture were concentrated on a porous polymer precolumn and analyzed by high-resolution gas chromatographic mass spectrometry. Twenty different compounds were identified. Both qualitative an...

The yeast two-hybrid system is a powerful tool to monitor protein-protein interactions. It relies on the expression of hybrid proteins in the yeast nucleus and the activation of reporter genes when a hybrid transcription factor (e.g., Gal4) is reconstituted via two interacting proteins (1). The method has been used at the genome level in organisms ranging from viruses to plants to assess protei...