The molecular basis of common variable immunodeficiency (CVID) is unknown. To assess humoral immunity in CVID, we selected 24 patients with early or late onset of disease. X-linked agammaglobulinemia (XLA), X-linked hyper-IgM syndrome (XHIM), and non-XHIM were excluded based on clinical phenotype, assessment of the immune response, presence of Bruton's tyrosine kinase (Btk) in monocytes or plat...

Since its inception by Svedberg and coworkers in the 1920s, the analytical ultracentrifuge has provided a powerful tool in biochemistry and molecular biology, with applications ranging from simple purity checks and particle shape determinations from sedimentation velocity, isolation and purification of macromolecules using density gradient techniques right through to the evaluation of mol wt an...

There are many types of tyrosine kinase involved in intracellular mitogenic mechanism, particularly, Tec tyrosine kinase are involved in apoptosis pathway. Tec tyrosine kinases do not have any transmembrane domain so it is known as non receptor tyrosine kinase. There are various types of Tec tyrosine kinase like Txk, Btk, Itk, Rik and Bmx. These kinases have different protein or domain structur...

X-linked agammaglobulinemia (XLA) is a rare genetic disorder caused by mutations in the Bruton's tyrosine kinase (BTK) gene. These mutations cause defects in early B cell development. A patient with no circulating B cells and low serum immunoglobulin isotypes was studied as were his mother and sister. Monocyte BTK protein expression was evaluated by flow cytometry. The mutation was determined u...

The state-of-the-art oxygen electrode materials used in solid oxide cells at 600–800 °C can deteriorate by gradual passivation of their surfaces related to changes the surface chemistry. We have recently observed unexpected recovery oxygen-exchange activity on La0.6Sr0.4FeO3 after short-term exposure high temperatures; a 10 h-long thermal treatment above 800 resulted 40-fold increase coefficien...

Returning to the University of Uppsala after 1 year in ON DECEMBER 1, 1996, the world lost one of this century’s giants in hematology. Jan Gosta WaldenMunich, he studied diseases associated with an elevation of the erythrocyte sedimentation rate (ESR). Waldenström ström was born the son and grandson of physicians in Stockholm, Sweden, on April 17, 1906. During his long and distindescribed three...

Taxol, 1-ß-D-arabinofuranosy Icytosine (ara-C), and etoposide induce apoptosis in HL-60 cells that is blocked by overexpression of Bcl-2 or Ikl-\,, A ~60-amino acid "loop"domain of Bcl-2 and Bcl-xLthat contains phosphorylation sites is known to negatively regulate their antiapoptotic function. In the present studies, Taxol-, ara-C-, or etoposide-induced apoptosis was examined in HL-60/Bcl-2A a...

primary immunodeficiency diseases (pid) consist of a group of long-term illnesses which had permanent psychiatric effects on the patients and their parents. this study was designed to find out the most important origins and aspects of stressor in parents of pid patients.to assess the  impact of  psychiatric aspects in parents  of pid  patients, a valid and reliable questionnaire was compiled ba...

A protease inhibitor has been purified from platelets by ammonium sulfate fractionation, QAE Sephadex chromatography, Sephadex G-200 gel filtration, and affinity chromatography on concanavalin A-Sepharose to apparent homogeneity on disc gel electrophoresis. The following properties of the inhibitor suggest its relationship to plasma a1 -antitrypsin: antigenic identity with a antitrypsin, molecu...

In the present study, we have addressed the role of the linker for activation of T cells (LAT) in the regulation of phospholipase Cgamma2 (PLCgamma2) by the platelet collagen receptor glycoprotein VI (GPVI). LAT is tyrosine phosphorylated in human platelets heavily in response to collagen, collagen-related peptide (CRP), and FcgammaRIIA cross-linking but only weakly in response to the G-protein...