A quantitative bio-imaging platform is developed for analysis of human cancer dissemination in a short-term vertebrate xenotransplantation assay. Six days after implantation of cancer cells in zebrafish embryos, automated imaging in 96 well plates coupled to image analysis algorithms quantifies spreading throughout the host. Findings in this model correlate with behavior in long-term rodent xen...

Osmotically stabilized Escherichia coli cells subjected to freezing and thawing were utilized as the source of enzymes for a peptidoglycan pathway assay that can be used to simultaneously test all targets of the committed steps of cell wall biosynthesis. The use of (14)C-labeled UDP-N-acetylglucosamine (UDP-GlcNAc) as a substrate allows the direct detection of cross-linked peptidoglycan formed....

A highly sensitive assay procedure for 5,5-diphenylhydantoin (DPH) involves oxidation with alkaline permanganate to form benzophenone. This is then extracted with heptane, allowed to react with concentrated sulfuric acid, and the fluorescence of the reaction product is measured. The procedure appears to be fairly specific for DPH; 5.(p.hyd roxyphenyl)-5.phenylhydantoin (a DPH metabolite), pheno...

Recent investigations concerning the significance of porphyrins in erythrocytes indicate a need for a rapid quantitative assay, easily adaptable to routine clinical use. To till this need, we have developed a simplified procedure for the measurement of porphyrins (essentially protoporphyrin) in whole blood. Blood is stirred with acetone-ethyl acetate and the porphyrins are extracted with formic...

Industrial revolution has intensified water pollution due to the indiscriminate discharge of untreated industrial effluents into bodies, posing a serious threat whole ecosystem. Recently, floating treatment wetlands system (FTWs) technique been used as one most innovative, cost-effective and environment-friendly option for wastewater treatment. The present study is aimed at investigating Malach...

Bilirubin (BR) is clinically confirmed as a biomarker for liver health and used to assess the prognosis of cirrhosis. Optical chemical methods have been utilized blood BR biosensing. While optical offer real-time monitoring are handy immune infection, measurements may not be practical due instrument complexity space requirements. This study investigated dual-wavelength (DWL) technique estimatio...