نتایج جستجو برای: when considering his habitus

تعداد نتایج: 1692680  

Hamid Reza Jalaeipour Michael Bitsch

The following paper is dealing with the topic of nations and nationalism. After giving a general overview of the mainstream work conducted regarding the national subject, the paper will in the first place focus on Bourdieu’s so called scholastic view which can be summarized as the bias between a scientific, theoretical reason and the practical reason that is applied by the social actors in the ...

Journal: :Microbiology 2003
M M Vickerman M Wang L J Baker

The Streptococcus gordonii glucosyltransferase structural gene, gtfG, is located immediately downstream from its positive transcriptional regulatory determinant, rgg. Recent genetic studies have indicated that the 3' end of rgg is involved either directly as a binding site or indirectly, e.g. by playing a role in secondary structure, in the interaction of Rgg with the gtfG promoter. A previousl...

Journal: :ACS nano 2010
Duane E Prasuhn Juan B Blanco-Canosa Gary J Vora James B Delehanty Kimihiro Susumu Bing C Mei Philip E Dawson Igor L Medintz

One of the principle hurdles to wider incorporation of semiconductor quantum dots (QDs) in biology is the lack of facile linkage chemistries to create different types of functional QD--bioconjugates. A two-step modular strategy for the presentation of biomolecules on CdSe/ZnS core/shell QDs is described here which utilizes a chemoselective, aniline-catalyzed hydrazone coupling chemistry to appe...

2013
Avinash S. Punekar Maria Selmer

Methyltransferase RlmJ uses the cofactor S-adenosylmethionine to methylate the exocyclic nitrogen N6 of nucleotide A2030 in 23S rRNA during ribosome assembly in Escherichia coli. RlmJ with a C-terminal hexahistidine tag was overexpressed in E. coli and purified as a monomer using Ni(2+)-affinity and size-exclusion chromatography. The recombinant RlmJ was crystallized using the sitting-drop vapo...

2013
Mark A. Arbing Sum Chan Liam Harris Emmeline Kuo Tina T. Zhou Christine J. Ahn Lin Nguyen Qixin He Jamie Lu Phuong T. Menchavez Annie Shin Thomas Holton Michael R. Sawaya Duilio Cascio David Eisenberg

The expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. The reason is that the solubility and proper folding of each subunit of the complex requires physical association with other subunits of the complex. The genomes of pathogenic mycobacteria encode many small protein complexes, implicated in bacterial fitness and pathogenicit...

Journal: :Biochemistry 2005
David M Duda Chingkuang Tu S Zoë Fisher Haiqain An Craig Yoshioka Lakshmanan Govindasamy Philip J Laipis Mavis Agbandje-McKenna David N Silverman Robert McKenna

The residue phenylalanine 198 (Phe 198) is a prominent cause of the lower activity of human carbonic anhydrase III (HCA III) compared with HCA II and other isozymes which have leucine at this site. We report the crystal structures of HCA III and the site-directed mutant F198L HCA III, both at 2.1 A resolution, and the enhancement of catalytic activity by exogenous proton donors containing imida...

Journal: :Scientific reports 2016
Veeraraghavan Usha Adrian J Lloyd David I Roper Christopher G Dowson Guennadi Kozlov Kalle Gehring Smita Chauhan Hasan T Imam Claudia A Blindauer Gurdyal S Besra

With the increased incidence of tuberculosis (TB) caused by Mycobacterium tuberculosis there is an urgent need for new and better anti-tubercular drugs. N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) is a key enzyme in the succinylase pathway for the biosynthesis of meso-diaminopimelic acid (meso-DAP) and L-lysine. DapE is a zinc containing metallohydrolase which hydrolyses N-succinyl ...

Journal: :Molecular human reproduction 2009
Jasmine Lefebvre Guy Boileau Puttaswamy Manjunath

Mammalian sperm undergo a series of maturation steps before acquiring fertilization competence. Our previous work demonstrated the importance of binder of sperm (BSP) proteins in bovine sperm capacitation. Recent studies identified a BSP-homologous DNA sequence in the human genome (BSPH1) and mRNA expression in the epididymis. The aim of this study was to develop an efficient method to express ...

Journal: :Plasmid 2008
C J Rocco K L Dennison Vadim A Klenchin I Rayment J C Escalante-Semerena

We describe the construction and use of two sets of vectors for the over-expression and purification of protein from Escherichia coli. The set of pTEV plasmids (pTEV3, 4, 5) directs the synthesis of a recombinant protein with a N-terminal hexahistidine (His(6)) tag that is removable by the tobacco etch virus (TEV) protease. The set of pKLD plasmids (pKLD66, 116) directs the synthesis of a recom...

Journal: :Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2010
D V Nascimento E M B Lemes J L S Queiroz J G Silva H J Nascimento E D Silva R Hirata A A S O Dias C S Santos G M B Pereira A L Mattos-Guaraldi G R G Armoa

The construction of a hexahistidine-tagged version of the B fragment of diphtheria toxin (DTB) represents an important step in the study of the biological properties of DTB because it will permit the production of pure recombinant DTB (rDTB) in less time and with higher yields than currently available. In the present study, the genomic DNA of the Corynebacterium diphtheriae Park Williams 8 (PW8...

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