نتایج جستجو برای: was amplified and sequenced using conventional rt

تعداد نتایج: 17375690  

Journal: :Journal of clinical microbiology 2006
W Allan Nix M Steven Oberste Mark A Pallansch

A reverse transcription-seminested PCR (RT-snPCR) assay was developed for the detection and identification of enterovirus (EV) RNA in clinical specimens. Three conserved protein motifs were identified by aligning the VP3 and VP1 sequences of prototype EV strains. Consensus degenerate primers were designed from a conserved VP3 motif and a distal VP1 motif for the first PCR. Consensus-degenerate ...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده علوم پایه 1391

food safety is considered as an important world concern. the detection of pathogen and toxic materials in food has an important role in public health care. in recent years, the applications of microbial biosensor have been developed as a new approach for toxicity control. luminescent microbial biosensor is an appropriate biological tool for protection of natural environment. this type of biosen...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تربیت مدرس 1389

growing demands and requires of high data rate systems cause significant increase of high frequency systems for wideband communication applications. as mixers are one of the main blocks of each receivers and its performance has great impact on receiver’s performance; in this thesis, a new solution for ku-band (12-18 ghz) mixer design in tsmc 0.18 µm is presented. this mixer has high linearity a...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و علوم انسانی 1391

the present dissertation aims to investigate four-word lexical bundles in applied linguistics research articles by iranian and internationally-published writers. the aims of this study are two-fold: first of all, attempts have been made to create a comprehensive list of the most commonly used four-word lexical bundles categorized by their type and token frequency, their structural characteristi...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده ادبیات و علوم انسانی دکتر علی شریعتی 1392

like any other learning activity, translation is a problem solving activity which involves executing parallel cognitive processes. the ability to think about these higher processes, plan, organize, monitor and evaluate the most influential executive cognitive processes is what flavell (1975) called “metacognition” which encompasses raising awareness of mental processes as well as using effectiv...

پایان نامه :وزارت علوم، تحقیقات و فناوری - پژوهشگاه شیمی و مهندسی شیمی ایران - پژوهشکده شیمی تجزیه و معدنی 1390

hydrochlorothiazide (hct) is a diuretic agent which is shown to be effective in the treatment of hypertension. literature reports have demonstrated that urinary excretion data may be used to assess the bioavailability of various formulations containing this thiazide. also hct consumption by the athletes is one of the drugs which should be regulated by world anti-doping agency (wada), because of...

Journal: :iranian journal of virology 0
h fani msc student, razi vaccine & serum research institute, karaj, iran m bakhshesh animal virology department, research and diagnosis, razi vaccine & serum research institute, karaj, iran f pourasgari department of biotechnology, research and diagnosis, razi vaccine & serum research institute, karaj, iran z sadigh department of human viral vaccine, research and production, razi vaccine & serum research institute, karaj, iran

background and aims: rotaviruses are the main cause of gastroenteritis in the young of human and a variety of animals in the world. rotavirus g6 and g10 are the most prevalent genotypes in cattle worldwide, but are also found in human with possible origination from animals. materials and methods: in this work, we have detected the g6 and g10 genotypes of bovine rotaviruses isolated from diarrhe...

Degenerate primers-based polymerase chain reaction (PCR) are commonly used for isolation of unidentified gene sequences in related organisms. For designing the degenerate primers, we propose the use of local alignment search method for searching the conserved regions long enough to design an acceptable primer pair. To test this method, a WD40 repeat-containing domain protein from Beauveria bass...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه سیستان و بلوچستان 1390

cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...

A. Ghalyanchilangeroudi, A. M. Hamdan A. Shayeganmehr F. S. Mousavi H. Hosseini M. H. Fallah Mehrabadi M. Jabbarifakhr M. S. Sediqian N. Sadri

Background: Avian infectious bronchitis (IB) is a highly contagious viral disease which affects the poultry industry. The virus exists in a wide variety of genotypes, and phylogenetic analysis has been used to classify infectious bronchitis virus (IBV) strains. Aims: The object of the study is a molecular characterization of circulating IBV in Afghanistan as a ...

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